Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic predisposition.
Bottom Line: For the micronucleus assay lymphocytes were exposed in vitro to 3.5 Gy (60)Co gamma-rays at a high dose rate or low dose rate. 70 h post-irradiation cultures were arrested and micronuclei were scored in 1000 binucleate cells.A higher proportion of the patients were radiosensitive with the micronucleus assay (45% with high dose rate and 61% with low dose rate).No correlation was found between the G2 and the G0-micronucleus chromosomal radiosensitivity.
Affiliation: Department of Anatomy, University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium.Show MeSH
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Mentions: In this study the G2 assay and MN (HDR/LDR) assay were carried out on blood samples collected from 62 breast cancer patients and 60 healthy women. However, not all the G2 and MN cultures set up in this study were successful. Only those samples from which we could score 50 metaphases for the presence of chromatid breaks or 1000 BN cells for MN analysis, in both the irradiated and sham-irradiated cultures, were included in the study. The numbers of successful samples obtained with the G2 and MN assay are given in Tables 1Table 1Mean values, standard deviations, ranges and percentage of radiosensitive breast cancer patients and controls and 2Table 2Mean values, standard deviations, ranges and percentage radiosensitivity of (1) breast cancer patients with a family history and a BRCA 1 or 2 mutation, (2) non-BRCA patients with a family history and (3) young breast cancer patients without a family history and Figures 1Figure 1
Affiliation: Department of Anatomy, University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium.