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15-PGJ2, but not thiazolidinediones, inhibits cell growth, induces apoptosis, and causes downregulation of Stat3 in human oral SCCa cells.

Nikitakis NG, Siavash H, Hebert C, Reynolds MA, Hamburger AW, Sauk JJ - Br. J. Cancer (2002)

Bottom Line: Interestingly, rosiglitazone and ciglitazone, two members of the thiazolidinedione family of PPARgamma activators, did not exert a growth inhibitory effect.In contrast, other PPARgamma did not induce similar effects.Our results provide the first evidence of significant antineoplastic effects of 15-PGJ(2) on human oral squamous cell carcinoma cells, which may be related to downmodulation of Stat3 and are at least partly mediated through PPARgamma-independent events.

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic Sciences and Pathology, University of Maryland, 666 W. Baltimore Street, Room 4-C-02, Baltimore, MD 21201, USA. nin001@dental.umaryland.edu

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(A, B) Immunocytochemical detection of PPARγ protein expression in oral SCCa cells (A, SCC25; B, SCC9). (C) Immunohistochemical detection of PPARγ in specimens of oral SCCa; immunostaining was limited to the well-differentiated areas of the tumours.
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fig1: (A, B) Immunocytochemical detection of PPARγ protein expression in oral SCCa cells (A, SCC25; B, SCC9). (C) Immunohistochemical detection of PPARγ in specimens of oral SCCa; immunostaining was limited to the well-differentiated areas of the tumours.

Mentions: Quantitative RT–PCR analysis showed mRNA expression for PPARγ in all four oral SCCa cell lines. The lowest levels of PPARγ mRNA were expressed in SCC25 and the highest in SCC9 (Table 1Table 1Relative PPARγ mRNA expression in oral SCCa cell linesa). Protein expression of PPARγ was detected by means of immunocytochemistry; PPARγ immunostaining, primarily in a cytoplasmic location, was evident in all four oral SCCa cell lines (Figure 1A,BFigure 1


15-PGJ2, but not thiazolidinediones, inhibits cell growth, induces apoptosis, and causes downregulation of Stat3 in human oral SCCa cells.

Nikitakis NG, Siavash H, Hebert C, Reynolds MA, Hamburger AW, Sauk JJ - Br. J. Cancer (2002)

(A, B) Immunocytochemical detection of PPARγ protein expression in oral SCCa cells (A, SCC25; B, SCC9). (C) Immunohistochemical detection of PPARγ in specimens of oral SCCa; immunostaining was limited to the well-differentiated areas of the tumours.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376283&req=5

fig1: (A, B) Immunocytochemical detection of PPARγ protein expression in oral SCCa cells (A, SCC25; B, SCC9). (C) Immunohistochemical detection of PPARγ in specimens of oral SCCa; immunostaining was limited to the well-differentiated areas of the tumours.
Mentions: Quantitative RT–PCR analysis showed mRNA expression for PPARγ in all four oral SCCa cell lines. The lowest levels of PPARγ mRNA were expressed in SCC25 and the highest in SCC9 (Table 1Table 1Relative PPARγ mRNA expression in oral SCCa cell linesa). Protein expression of PPARγ was detected by means of immunocytochemistry; PPARγ immunostaining, primarily in a cytoplasmic location, was evident in all four oral SCCa cell lines (Figure 1A,BFigure 1

Bottom Line: Interestingly, rosiglitazone and ciglitazone, two members of the thiazolidinedione family of PPARgamma activators, did not exert a growth inhibitory effect.In contrast, other PPARgamma did not induce similar effects.Our results provide the first evidence of significant antineoplastic effects of 15-PGJ(2) on human oral squamous cell carcinoma cells, which may be related to downmodulation of Stat3 and are at least partly mediated through PPARgamma-independent events.

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic Sciences and Pathology, University of Maryland, 666 W. Baltimore Street, Room 4-C-02, Baltimore, MD 21201, USA. nin001@dental.umaryland.edu

Show MeSH
Related in: MedlinePlus