Nifedipine improves blood flow and oxygen supply, but not steady-state oxygenation of tumours in perfusion pressure-controlled isolated limb perfusion.
Bottom Line: Perfusion was performed by cannulation of the femoral artery and vein in rats bearing DS-sarcoma on the hind foot dorsum.Following equilibration, nifedipine was continuously infused for 30 min (8.3 microg min(-1) kg(-1) BW).Despite the increased oxygen availability (+63%) seen upon application of this calcium channel blocker, nifedipine does not result in a substantial reduction of tumour hypoxia, most probably due to an increase in O(2) uptake with rising O(2) supply to the tumour-bearing hind limb.
Affiliation: Institute of Physiology and Pathophysiology, University of Mainz, Duesbergweg 6, 55099 Mainz, Germany. OLTHEWS@uni-mainz.deShow MeSH
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Mentions: A single-pass ILP of the tumour-bearing extremity was performed (in contrast to the earlier attempts of Nagel et al (1987) where a closed blood circuit was used) using a perfusion system consisting of a peristaltic roller pump (mp13GJ-4, Ismatec, Zürich, Switzerland), a capillary oxygenator (SPS40002-P, Fresenius, Bad Homburg, Germany), a water-filled heat exchanger used to maintain a perfusate blood temperature of 36.5 to 37.5°C, polyethylene cannulas and silicone tubing (Figure 1Figure 1
Affiliation: Institute of Physiology and Pathophysiology, University of Mainz, Duesbergweg 6, 55099 Mainz, Germany. OLTHEWS@uni-mainz.de