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Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1.

Ameri K, Burke B, Lewis CE, Harris AL - Br. J. Cancer (2002)

Bottom Line: Novel approaches to cancer gene therapy currently exploit tumour hypoxia to achieve transcriptional targeting using oxygen-regulated enhancer elements called hypoxia response elements.Mutational analysis demonstrated that the base immediately 5' to this modulates the anoxic/hypoxic induction of the secondary anoxia response element, such that TACGTG>GACGTG>CACGTG.A similar correlation was found for erythropoietin, phosphoglycerate kinase 1, and aldolase hypoxia response elements, which contain these respective 5' flanking bases.

View Article: PubMed Central - PubMed

Affiliation: Tumour Targeting Group, Division of Genomic Medicine, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, UK.

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Immunoblot analysis of HIF-1α, HIF-1β, EPAS 1, and ATF-1 in MCF-7 cells following exposure to 0, 0.5, 1, and 21% O2 (normoxia) for 16 h. Blots were stripped and re-probed for β-actin as a loading control; no differences were observed (data not shown).
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fig5: Immunoblot analysis of HIF-1α, HIF-1β, EPAS 1, and ATF-1 in MCF-7 cells following exposure to 0, 0.5, 1, and 21% O2 (normoxia) for 16 h. Blots were stripped and re-probed for β-actin as a loading control; no differences were observed (data not shown).

Mentions: Low levels of HIF-1α and HIF-1β were detectable in normoxic MCF-7 nuclear extracts. No HIF-2α (EPAS 1) was detected in normoxia. Levels of all three of these proteins increased markedly in hypoxia (1% and 0.5% O2) and anoxia (Figure 5Figure 5


Regulation of a rat VL30 element in human breast cancer cells in hypoxia and anoxia: role of HIF-1.

Ameri K, Burke B, Lewis CE, Harris AL - Br. J. Cancer (2002)

Immunoblot analysis of HIF-1α, HIF-1β, EPAS 1, and ATF-1 in MCF-7 cells following exposure to 0, 0.5, 1, and 21% O2 (normoxia) for 16 h. Blots were stripped and re-probed for β-actin as a loading control; no differences were observed (data not shown).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376195&req=5

fig5: Immunoblot analysis of HIF-1α, HIF-1β, EPAS 1, and ATF-1 in MCF-7 cells following exposure to 0, 0.5, 1, and 21% O2 (normoxia) for 16 h. Blots were stripped and re-probed for β-actin as a loading control; no differences were observed (data not shown).
Mentions: Low levels of HIF-1α and HIF-1β were detectable in normoxic MCF-7 nuclear extracts. No HIF-2α (EPAS 1) was detected in normoxia. Levels of all three of these proteins increased markedly in hypoxia (1% and 0.5% O2) and anoxia (Figure 5Figure 5

Bottom Line: Novel approaches to cancer gene therapy currently exploit tumour hypoxia to achieve transcriptional targeting using oxygen-regulated enhancer elements called hypoxia response elements.Mutational analysis demonstrated that the base immediately 5' to this modulates the anoxic/hypoxic induction of the secondary anoxia response element, such that TACGTG>GACGTG>CACGTG.A similar correlation was found for erythropoietin, phosphoglycerate kinase 1, and aldolase hypoxia response elements, which contain these respective 5' flanking bases.

View Article: PubMed Central - PubMed

Affiliation: Tumour Targeting Group, Division of Genomic Medicine, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, UK.

Show MeSH
Related in: MedlinePlus