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Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites.

Sakakura C, Hagiwara A, Nakanishi M, Shimomura K, Takagi T, Yasuoka R, Fujita Y, Abe T, Ichikawa Y, Takahashi S, Ishikawa T, Nishizuka I, Morita T, Shimada H, Okazaki Y, Hayashizaki Y, Yamagishi H - Br. J. Cancer (2002)

Bottom Line: Our examinations of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB showed that 24 genes were up-regulated and 17 genes down-regulated besides expression sequence tags.Reverse transcriptase-polymerase chain reaction confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites.The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination, promise to provide a new insight into the study of human gastric cancer peritoneal dissemination.

View Article: PubMed Central - PubMed

Affiliation: Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kawaramachi-dori, Kyoto 602-8566, Japan. sakakura@koto.kpu-m.ac.jp

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High-density cDNA microarray analysis. Validation in expression of selected genes from 21 68 clones in six gastric cancer cell lines. Data are represented in a matrix format: each row represents a single gene, and each column an experimental sample. In each sample, the ratio of the number of transcripts of each gene to the medium abundance of the gene's transcript among all the cell lines is represented by the colour of the corresponding cell in the matrix. Green square,transcript level below the median; black squares, transcript levels equal to the medium; red squares, transcript levels greater than the median. Colour saturation reflects the magnitude of the ratio relative to the median for each set of samples. (A) Cluster analysis of selected genes of six gastric cancer cell lines using the RIKEN cDNA microarray. Bars to the right identify the location of the inserts displayed in panels B and C. (B) Up-regulated genes in gastric cancer cells from malignant ascites. (C) Down-regulated genes in gastric cancer cells from malignant ascites
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fig1: High-density cDNA microarray analysis. Validation in expression of selected genes from 21 68 clones in six gastric cancer cell lines. Data are represented in a matrix format: each row represents a single gene, and each column an experimental sample. In each sample, the ratio of the number of transcripts of each gene to the medium abundance of the gene's transcript among all the cell lines is represented by the colour of the corresponding cell in the matrix. Green square,transcript level below the median; black squares, transcript levels equal to the medium; red squares, transcript levels greater than the median. Colour saturation reflects the magnitude of the ratio relative to the median for each set of samples. (A) Cluster analysis of selected genes of six gastric cancer cell lines using the RIKEN cDNA microarray. Bars to the right identify the location of the inserts displayed in panels B and C. (B) Up-regulated genes in gastric cancer cells from malignant ascites. (C) Down-regulated genes in gastric cancer cells from malignant ascites

Mentions: We performed a global analysis of gene expression of 21 168 genes in SNU-1, SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB cells using a high-density cDNA microarray and compared the gene expression profiles of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB cells with that of SNU-1. To eliminate data with low reliability, genes whose expression was regarded as absent in both cell lines as a result of software analysis were excluded. The results of the analysis by TREEVIEW are shown in Figure 1Figure 1


Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites.

Sakakura C, Hagiwara A, Nakanishi M, Shimomura K, Takagi T, Yasuoka R, Fujita Y, Abe T, Ichikawa Y, Takahashi S, Ishikawa T, Nishizuka I, Morita T, Shimada H, Okazaki Y, Hayashizaki Y, Yamagishi H - Br. J. Cancer (2002)

High-density cDNA microarray analysis. Validation in expression of selected genes from 21 68 clones in six gastric cancer cell lines. Data are represented in a matrix format: each row represents a single gene, and each column an experimental sample. In each sample, the ratio of the number of transcripts of each gene to the medium abundance of the gene's transcript among all the cell lines is represented by the colour of the corresponding cell in the matrix. Green square,transcript level below the median; black squares, transcript levels equal to the medium; red squares, transcript levels greater than the median. Colour saturation reflects the magnitude of the ratio relative to the median for each set of samples. (A) Cluster analysis of selected genes of six gastric cancer cell lines using the RIKEN cDNA microarray. Bars to the right identify the location of the inserts displayed in panels B and C. (B) Up-regulated genes in gastric cancer cells from malignant ascites. (C) Down-regulated genes in gastric cancer cells from malignant ascites
© Copyright Policy
Related In: Results  -  Collection

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fig1: High-density cDNA microarray analysis. Validation in expression of selected genes from 21 68 clones in six gastric cancer cell lines. Data are represented in a matrix format: each row represents a single gene, and each column an experimental sample. In each sample, the ratio of the number of transcripts of each gene to the medium abundance of the gene's transcript among all the cell lines is represented by the colour of the corresponding cell in the matrix. Green square,transcript level below the median; black squares, transcript levels equal to the medium; red squares, transcript levels greater than the median. Colour saturation reflects the magnitude of the ratio relative to the median for each set of samples. (A) Cluster analysis of selected genes of six gastric cancer cell lines using the RIKEN cDNA microarray. Bars to the right identify the location of the inserts displayed in panels B and C. (B) Up-regulated genes in gastric cancer cells from malignant ascites. (C) Down-regulated genes in gastric cancer cells from malignant ascites
Mentions: We performed a global analysis of gene expression of 21 168 genes in SNU-1, SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB cells using a high-density cDNA microarray and compared the gene expression profiles of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB cells with that of SNU-1. To eliminate data with low reliability, genes whose expression was regarded as absent in both cell lines as a result of software analysis were excluded. The results of the analysis by TREEVIEW are shown in Figure 1Figure 1

Bottom Line: Our examinations of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB showed that 24 genes were up-regulated and 17 genes down-regulated besides expression sequence tags.Reverse transcriptase-polymerase chain reaction confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites.The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination, promise to provide a new insight into the study of human gastric cancer peritoneal dissemination.

View Article: PubMed Central - PubMed

Affiliation: Department of Digestive Surgery, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kawaramachi-dori, Kyoto 602-8566, Japan. sakakura@koto.kpu-m.ac.jp

Show MeSH
Related in: MedlinePlus