Elevated c-Src is linked to altered cell-matrix adhesion rather than proliferation in KM12C human colorectal cancer cells.
Bottom Line: Elevated expression and/or activity of c-Src, the prototype of the Src family of protein tyrosine kinases, is associated with the development of human colon cancer.Although elevated Src correlates with ability to metastasise to the liver after intrasplenic injection, we found that this was not linked to enhanced growth, either in vitro or in vivo as sub-cutaneous tumours.However, elevated Src was associated with enhanced attachment to extracellular matrix.
Affiliation: Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK.Show MeSH
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Mentions: (A) c-Src expression and activity (monitored by auto-phosphorylation at tyrosine-416) in KM12C cell clones (2C3 and 2C4) stably expressing active c-SrcY527F, or vector control (2CV), was examined and compared with parental KM12C cells and their metastatic derivatives, KM12SM and KM12L4A. Paxillin phosphorylation in vector- and c-SrcY527F-expressing KM12C cells was also examined. (B) In vitro growth of KM12C cells stably expressing active c-SrcY527F (2C3 and 2C4; seeded at 1×105 cells in 35 mm dishes) was compared with vector-transfected cells (2CV). (C) The doubling times of subcutaneous tumours formed after injection of 106 KM12C cells transfected with either vector (2CV) or active c-SrcY527F (2C3 and 2C4) were determined.
Affiliation: Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK.