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Elevated c-Src is linked to altered cell-matrix adhesion rather than proliferation in KM12C human colorectal cancer cells.

Jones RJ, Avizienyte E, Wyke AW, Owens DW, Brunton VG, Frame MC - Br. J. Cancer (2002)

Bottom Line: Elevated expression and/or activity of c-Src, the prototype of the Src family of protein tyrosine kinases, is associated with the development of human colon cancer.Although elevated Src correlates with ability to metastasise to the liver after intrasplenic injection, we found that this was not linked to enhanced growth, either in vitro or in vivo as sub-cutaneous tumours.However, elevated Src was associated with enhanced attachment to extracellular matrix.

View Article: PubMed Central - PubMed

Affiliation: Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK.

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(A) Immunofluorescence staining (anti-vinculin) of KM12C and KM12L4A cells grown under normal culture conditions. Visualisation was by reaction of primary antibody with FITC-conjugated secondary antibody and confocal microscopy. Bars are 100 μm. (B) Anti-vinculin staining of KM12C and KM12L4A cells after plating suspended cells on to poly-L-lysine (left panels) or fibronectin (right panels) for 60 min. Bars are 100 μm. Similar images to those shown for KM12L4A cells were obtained for KM12SM cells. (C) The ability of KM12C, KM12SM and KM12L4A cells to attach to different matrix components was monitored using Chromium-51-labelled cells that were plated on to collagen, fibronectin, laminin or vitronectin for 45 min at 37°C. A known number of cells in suspension was also counted to calibrate the Chromium-51 counts per cell, allowing estimation of the number of attached cells. The means and standard errors of three replicates are presented.
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fig3: (A) Immunofluorescence staining (anti-vinculin) of KM12C and KM12L4A cells grown under normal culture conditions. Visualisation was by reaction of primary antibody with FITC-conjugated secondary antibody and confocal microscopy. Bars are 100 μm. (B) Anti-vinculin staining of KM12C and KM12L4A cells after plating suspended cells on to poly-L-lysine (left panels) or fibronectin (right panels) for 60 min. Bars are 100 μm. Similar images to those shown for KM12L4A cells were obtained for KM12SM cells. (C) The ability of KM12C, KM12SM and KM12L4A cells to attach to different matrix components was monitored using Chromium-51-labelled cells that were plated on to collagen, fibronectin, laminin or vitronectin for 45 min at 37°C. A known number of cells in suspension was also counted to calibrate the Chromium-51 counts per cell, allowing estimation of the number of attached cells. The means and standard errors of three replicates are presented.

Mentions: As well as growth responses in fibroblasts (reviewed in Abram and Courtneidge, 2000), SFKs also influence cell adhesion in both fibroblasts (Fincham and Frame, 1998) and osteoclasts (Schwartzberg et al, 1997). We found that cultures of KM12L4A metastatic cells contained substantially more vinculin-containing peripheral adhesion structures than their KM12C non-metastatic counterparts under normal growth conditions (Figure 3AFigure 3


Elevated c-Src is linked to altered cell-matrix adhesion rather than proliferation in KM12C human colorectal cancer cells.

Jones RJ, Avizienyte E, Wyke AW, Owens DW, Brunton VG, Frame MC - Br. J. Cancer (2002)

(A) Immunofluorescence staining (anti-vinculin) of KM12C and KM12L4A cells grown under normal culture conditions. Visualisation was by reaction of primary antibody with FITC-conjugated secondary antibody and confocal microscopy. Bars are 100 μm. (B) Anti-vinculin staining of KM12C and KM12L4A cells after plating suspended cells on to poly-L-lysine (left panels) or fibronectin (right panels) for 60 min. Bars are 100 μm. Similar images to those shown for KM12L4A cells were obtained for KM12SM cells. (C) The ability of KM12C, KM12SM and KM12L4A cells to attach to different matrix components was monitored using Chromium-51-labelled cells that were plated on to collagen, fibronectin, laminin or vitronectin for 45 min at 37°C. A known number of cells in suspension was also counted to calibrate the Chromium-51 counts per cell, allowing estimation of the number of attached cells. The means and standard errors of three replicates are presented.
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Related In: Results  -  Collection

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fig3: (A) Immunofluorescence staining (anti-vinculin) of KM12C and KM12L4A cells grown under normal culture conditions. Visualisation was by reaction of primary antibody with FITC-conjugated secondary antibody and confocal microscopy. Bars are 100 μm. (B) Anti-vinculin staining of KM12C and KM12L4A cells after plating suspended cells on to poly-L-lysine (left panels) or fibronectin (right panels) for 60 min. Bars are 100 μm. Similar images to those shown for KM12L4A cells were obtained for KM12SM cells. (C) The ability of KM12C, KM12SM and KM12L4A cells to attach to different matrix components was monitored using Chromium-51-labelled cells that were plated on to collagen, fibronectin, laminin or vitronectin for 45 min at 37°C. A known number of cells in suspension was also counted to calibrate the Chromium-51 counts per cell, allowing estimation of the number of attached cells. The means and standard errors of three replicates are presented.
Mentions: As well as growth responses in fibroblasts (reviewed in Abram and Courtneidge, 2000), SFKs also influence cell adhesion in both fibroblasts (Fincham and Frame, 1998) and osteoclasts (Schwartzberg et al, 1997). We found that cultures of KM12L4A metastatic cells contained substantially more vinculin-containing peripheral adhesion structures than their KM12C non-metastatic counterparts under normal growth conditions (Figure 3AFigure 3

Bottom Line: Elevated expression and/or activity of c-Src, the prototype of the Src family of protein tyrosine kinases, is associated with the development of human colon cancer.Although elevated Src correlates with ability to metastasise to the liver after intrasplenic injection, we found that this was not linked to enhanced growth, either in vitro or in vivo as sub-cutaneous tumours.However, elevated Src was associated with enhanced attachment to extracellular matrix.

View Article: PubMed Central - PubMed

Affiliation: Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland, UK.

Show MeSH
Related in: MedlinePlus