Secretion of soluble complement inhibitors factor H and factor H-like protein (FHL-1) by ovarian tumour cells.
Bottom Line: We observed that the soluble complement regulators factor H and factor H-like protein were abundantly present in ascites samples as well as in primary tumours of patients with ovarian cancer.In ascites samples the mean level of factor H-like protein (130+/-55 microg ml(-1)) was 5.5-fold higher than in normal human serum (24+/-3 microg ml(-1)).Subsequently, the PA-1 and SW626 cell lines were found to secrete a soluble form of the membrane cofactor protein (CD46).
Affiliation: Department of Bacteriology and Immunology, Haartman Institute, University Central Hospital, FIN-0014 Helsinki, Finland.Show MeSH
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Mentions: The immunohistochemical evidence of tumour-associated factor H/FHL-1 suggested that the ovarian tumour cells could carry surface components that bind factor H and/or FHL-1, proteins which both have binding sites for glycosaminoglycans and other polyanions. In accordance, we found that all the ovarian tumour cell lines SK-OV-3, Caov-3, PA-1 and SW626 bound both 125I-labelled factor H and FHL-1 to their cell surfaces (Figure 4AFigure 4
Affiliation: Department of Bacteriology and Immunology, Haartman Institute, University Central Hospital, FIN-0014 Helsinki, Finland.