Secretion of soluble complement inhibitors factor H and factor H-like protein (FHL-1) by ovarian tumour cells.
Bottom Line: We observed that the soluble complement regulators factor H and factor H-like protein were abundantly present in ascites samples as well as in primary tumours of patients with ovarian cancer.In ascites samples the mean level of factor H-like protein (130+/-55 microg ml(-1)) was 5.5-fold higher than in normal human serum (24+/-3 microg ml(-1)).Subsequently, the PA-1 and SW626 cell lines were found to secrete a soluble form of the membrane cofactor protein (CD46).
Affiliation: Department of Bacteriology and Immunology, Haartman Institute, University Central Hospital, FIN-0014 Helsinki, Finland.Show MeSH
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Mentions: To investigate the presence of FHL-1 and factor H in ovarian tumours in vivo, we analysed cryostat sections of serous ovarian tumours with the VIG8 (detects SCR19-20 of factor H) and 196X (detects SCR1 of factor H and FHL-1) mAbs and immunoperoxidase staining. Examples of the stainings are shown in Figure 2Figure 2
Affiliation: Department of Bacteriology and Immunology, Haartman Institute, University Central Hospital, FIN-0014 Helsinki, Finland.