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Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.

Mooney LM, Al-Sakkaf KA, Brown BL, Dobson PR - Br. J. Cancer (2002)

Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.

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Alterations of the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells in response to STS. (A) Cells were treated with 1 μM STS for the times indicated. Cells were then stained with 40 nM DiOC6(3). Representative histoplots are shown for each time point. The dotted line separates cells with high and low fluorescence. (B) Caspase independent ΔΨm collapse. Cells were incubated with 100 μM z-VAD-fmk (Z-VAD) prior to 1 μM STS for 6 h. Mean fluorescence (FL-1) representing ΔΨm was measured by Flow Cytometry. Error bars represent the mean±s.e.m. of four independent experiments.
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fig5: Alterations of the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells in response to STS. (A) Cells were treated with 1 μM STS for the times indicated. Cells were then stained with 40 nM DiOC6(3). Representative histoplots are shown for each time point. The dotted line separates cells with high and low fluorescence. (B) Caspase independent ΔΨm collapse. Cells were incubated with 100 μM z-VAD-fmk (Z-VAD) prior to 1 μM STS for 6 h. Mean fluorescence (FL-1) representing ΔΨm was measured by Flow Cytometry. Error bars represent the mean±s.e.m. of four independent experiments.

Mentions: Alterations in the mitochondrial transmembrane potential (ΔΨm) have been shown to be important for the release of mitochondrial proteins such as cytochrome c, which when in the cytosol can lead to activation of the caspase cascade and subsequent death (Reed, 1997). We measured the mitochondrial transmembrane potential (ΔΨm) by staining with 40 nM DiOC6(3) after treatment with 1 μM STS. STS caused a time-dependent reduction in the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells (Figure 5AFigure 5


Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.

Mooney LM, Al-Sakkaf KA, Brown BL, Dobson PR - Br. J. Cancer (2002)

Alterations of the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells in response to STS. (A) Cells were treated with 1 μM STS for the times indicated. Cells were then stained with 40 nM DiOC6(3). Representative histoplots are shown for each time point. The dotted line separates cells with high and low fluorescence. (B) Caspase independent ΔΨm collapse. Cells were incubated with 100 μM z-VAD-fmk (Z-VAD) prior to 1 μM STS for 6 h. Mean fluorescence (FL-1) representing ΔΨm was measured by Flow Cytometry. Error bars represent the mean±s.e.m. of four independent experiments.
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Related In: Results  -  Collection

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fig5: Alterations of the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells in response to STS. (A) Cells were treated with 1 μM STS for the times indicated. Cells were then stained with 40 nM DiOC6(3). Representative histoplots are shown for each time point. The dotted line separates cells with high and low fluorescence. (B) Caspase independent ΔΨm collapse. Cells were incubated with 100 μM z-VAD-fmk (Z-VAD) prior to 1 μM STS for 6 h. Mean fluorescence (FL-1) representing ΔΨm was measured by Flow Cytometry. Error bars represent the mean±s.e.m. of four independent experiments.
Mentions: Alterations in the mitochondrial transmembrane potential (ΔΨm) have been shown to be important for the release of mitochondrial proteins such as cytochrome c, which when in the cytosol can lead to activation of the caspase cascade and subsequent death (Reed, 1997). We measured the mitochondrial transmembrane potential (ΔΨm) by staining with 40 nM DiOC6(3) after treatment with 1 μM STS. STS caused a time-dependent reduction in the mitochondrial transmembrane potential (ΔΨm) in MCF-7 and T47D cells (Figure 5AFigure 5

Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.

Show MeSH
Related in: MedlinePlus