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Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.

Mooney LM, Al-Sakkaf KA, Brown BL, Dobson PR - Br. J. Cancer (2002)

Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.

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Staurosporine induced PARP cleavage in MCF-7 and T47D cells. Both (A) MCF-7 and (B) T47D cells were either treated with 1 μM STS for the times indicated or pre-incubated with 100 μM z-VAD-fmk for 1 h prior to STS treatments. Cell lysates (40 μg protein) were prepared and samples subjected to 8% SDS–PAGE followed by Western transfer and probed with PARP antibody (full length protein is 113 kDa, and cleaved fragment is 85 kDa). The results are representative of three independent experiments.
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fig4: Staurosporine induced PARP cleavage in MCF-7 and T47D cells. Both (A) MCF-7 and (B) T47D cells were either treated with 1 μM STS for the times indicated or pre-incubated with 100 μM z-VAD-fmk for 1 h prior to STS treatments. Cell lysates (40 μg protein) were prepared and samples subjected to 8% SDS–PAGE followed by Western transfer and probed with PARP antibody (full length protein is 113 kDa, and cleaved fragment is 85 kDa). The results are representative of three independent experiments.

Mentions: Activation of the caspase cascade leads to cleavage of cellular proteins such as the DNA repair enzyme poly-ADP-ribose polymerase (PARP); cleavage results in inactivation of the enzyme. STS induced PARP cleavage in a time-dependent manner in MCF-7 cells; the 85 kDa cleavage product was observed as early as 3 h after treatment and this increased dramatically by 16 h (Figure 4AFigure 4


Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.

Mooney LM, Al-Sakkaf KA, Brown BL, Dobson PR - Br. J. Cancer (2002)

Staurosporine induced PARP cleavage in MCF-7 and T47D cells. Both (A) MCF-7 and (B) T47D cells were either treated with 1 μM STS for the times indicated or pre-incubated with 100 μM z-VAD-fmk for 1 h prior to STS treatments. Cell lysates (40 μg protein) were prepared and samples subjected to 8% SDS–PAGE followed by Western transfer and probed with PARP antibody (full length protein is 113 kDa, and cleaved fragment is 85 kDa). The results are representative of three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376174&req=5

fig4: Staurosporine induced PARP cleavage in MCF-7 and T47D cells. Both (A) MCF-7 and (B) T47D cells were either treated with 1 μM STS for the times indicated or pre-incubated with 100 μM z-VAD-fmk for 1 h prior to STS treatments. Cell lysates (40 μg protein) were prepared and samples subjected to 8% SDS–PAGE followed by Western transfer and probed with PARP antibody (full length protein is 113 kDa, and cleaved fragment is 85 kDa). The results are representative of three independent experiments.
Mentions: Activation of the caspase cascade leads to cleavage of cellular proteins such as the DNA repair enzyme poly-ADP-ribose polymerase (PARP); cleavage results in inactivation of the enzyme. STS induced PARP cleavage in a time-dependent manner in MCF-7 cells; the 85 kDa cleavage product was observed as early as 3 h after treatment and this increased dramatically by 16 h (Figure 4AFigure 4

Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.

View Article: PubMed Central - PubMed

Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.

Show MeSH
Related in: MedlinePlus