Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.
Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.
Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.Show MeSH
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Mentions: Activation of the caspase cascade leads to cleavage of cellular proteins such as the DNA repair enzyme poly-ADP-ribose polymerase (PARP); cleavage results in inactivation of the enzyme. STS induced PARP cleavage in a time-dependent manner in MCF-7 cells; the 85 kDa cleavage product was observed as early as 3 h after treatment and this increased dramatically by 16 h (Figure 4AFigure 4
Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.