Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells.
Bottom Line: MCF-7 cells did not express caspase-3, suggesting that DNA fragmentation occurred in the absence of caspase-3 and that other caspases may be involved.In addition, a time dependent and caspase-independent reduction of the mitochondrial transmembrane potential was observed; which appeared to occur after the release of cytochrome c.Apoptotic events in both cell types differ temporally, involving activation of different caspases and mitochondrial changes.
Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.Show MeSH
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Mentions: Studies of apoptosis, as determined by DNA fragmentation (ISNT), demonstrated that T47D and MCF-7 cells, when exposed to STS (0.2–1 μM), exhibited a concentration-dependent increase in apoptosis, with maximal effects for both cells being at 1 μM STS (data not shown). Apoptosis was also a time-dependent process. When exposed to 1 μM STS, a significant increase in cells displaying DNA fragmentation was evident at 14 h in T47D cells, yet apoptotic changes were detected as early as 4 h in MCF-7 cells (Figure 1AFigure 1
Affiliation: Institute for Cancer Studies, Division of Genomic Medicine, Medical School, University of Sheffield, Sheffield S10 2RX, UK.