Binding of TGF-beta1 latency-associated peptide (LAP) to alpha(v)beta6 integrin modulates behaviour of squamous carcinoma cells.
Bottom Line: Since TGF-beta1 is present in squamous carcinomas, it is possible that latency associated peptide may modulate malignant keratinocyte behaviour independently from the classical TGF-beta signalling pathways through its interaction with integrins.We show here that when latency associated peptide is immobilised onto a surface, it acts as an alpha(v)beta6-specific ligand for oral squamous carcinoma cells promoting adhesion and haptotactic migration in addition to alpha(v)beta6-dependent increase in pro-MMP-9 expression.In contrast, even very low concentrations of soluble latency associated peptide (0.1 microg ml(-1)) inhibited alpha(v)beta6-dependent adhesion, migration and invasion.
Affiliation: Department of Oral Pathology, Eastman Dental Institute, University College London, UK.Show MeSH
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Mentions: VB6 cells show increased αvβ6-dependent migration towards fibronectin relative to the other cell lines (Thomas et al, 2001b). To determine whether VB6 cells migrated towards LAP, haptotactic migration assays were performed using LAP-coated Transwell filters. VB6 cells migrated less well towards LAP than fibronectin (a relative reduction of 53% over four experiments; P=0.039; data not shown). However, migration towards LAP was increased significantly in VB6 cells compared with C1 cells (P=<0.001; (Figure 3AFigure 3
Affiliation: Department of Oral Pathology, Eastman Dental Institute, University College London, UK.