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Binding of TGF-beta1 latency-associated peptide (LAP) to alpha(v)beta6 integrin modulates behaviour of squamous carcinoma cells.

Thomas GJ, Hart IR, Speight PM, Marshall JF - Br. J. Cancer (2002)

Bottom Line: Since TGF-beta1 is present in squamous carcinomas, it is possible that latency associated peptide may modulate malignant keratinocyte behaviour independently from the classical TGF-beta signalling pathways through its interaction with integrins.We show here that when latency associated peptide is immobilised onto a surface, it acts as an alpha(v)beta6-specific ligand for oral squamous carcinoma cells promoting adhesion and haptotactic migration in addition to alpha(v)beta6-dependent increase in pro-MMP-9 expression.In contrast, even very low concentrations of soluble latency associated peptide (0.1 microg ml(-1)) inhibited alpha(v)beta6-dependent adhesion, migration and invasion.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Eastman Dental Institute, University College London, UK.

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Flow cytometric analysis of integrin expression by cell lines. The geometric mean fluorescence (arbitrary units, log scale) as measured by flow cytometry of cells labelled with anti-integrin antibodies is shown. Negative control had secondary antibody only and has been subtracted from the results. Figure shows a representative experiment (ND=not detected). Flow cytometry confirmed high αvβ6 expression by VB6 cells and that C1  transfectant cells express low levels of endogenous αvβ6. H357 cells are αv- negative. The cell lines express similar levels of β1 integrins.
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fig1: Flow cytometric analysis of integrin expression by cell lines. The geometric mean fluorescence (arbitrary units, log scale) as measured by flow cytometry of cells labelled with anti-integrin antibodies is shown. Negative control had secondary antibody only and has been subtracted from the results. Figure shows a representative experiment (ND=not detected). Flow cytometry confirmed high αvβ6 expression by VB6 cells and that C1 transfectant cells express low levels of endogenous αvβ6. H357 cells are αv- negative. The cell lines express similar levels of β1 integrins.

Mentions: Figure 1Figure 1


Binding of TGF-beta1 latency-associated peptide (LAP) to alpha(v)beta6 integrin modulates behaviour of squamous carcinoma cells.

Thomas GJ, Hart IR, Speight PM, Marshall JF - Br. J. Cancer (2002)

Flow cytometric analysis of integrin expression by cell lines. The geometric mean fluorescence (arbitrary units, log scale) as measured by flow cytometry of cells labelled with anti-integrin antibodies is shown. Negative control had secondary antibody only and has been subtracted from the results. Figure shows a representative experiment (ND=not detected). Flow cytometry confirmed high αvβ6 expression by VB6 cells and that C1  transfectant cells express low levels of endogenous αvβ6. H357 cells are αv- negative. The cell lines express similar levels of β1 integrins.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376166&req=5

fig1: Flow cytometric analysis of integrin expression by cell lines. The geometric mean fluorescence (arbitrary units, log scale) as measured by flow cytometry of cells labelled with anti-integrin antibodies is shown. Negative control had secondary antibody only and has been subtracted from the results. Figure shows a representative experiment (ND=not detected). Flow cytometry confirmed high αvβ6 expression by VB6 cells and that C1 transfectant cells express low levels of endogenous αvβ6. H357 cells are αv- negative. The cell lines express similar levels of β1 integrins.
Mentions: Figure 1Figure 1

Bottom Line: Since TGF-beta1 is present in squamous carcinomas, it is possible that latency associated peptide may modulate malignant keratinocyte behaviour independently from the classical TGF-beta signalling pathways through its interaction with integrins.We show here that when latency associated peptide is immobilised onto a surface, it acts as an alpha(v)beta6-specific ligand for oral squamous carcinoma cells promoting adhesion and haptotactic migration in addition to alpha(v)beta6-dependent increase in pro-MMP-9 expression.In contrast, even very low concentrations of soluble latency associated peptide (0.1 microg ml(-1)) inhibited alpha(v)beta6-dependent adhesion, migration and invasion.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology, Eastman Dental Institute, University College London, UK.

Show MeSH
Related in: MedlinePlus