Hyperstable U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells.
Bottom Line: In this study, we utilised U1 small nuclear RNA (snRNA) that binds physiologically to the 5' splice site (5'ss) of pre-mRNA, to develop a novel vector system that permits imposed binding of antisense RNA to its target.This revealed that two of the hyperstable U1snRNAs induced cell death after gene transduction, and significantly reduced the number of G418-resistant colonies to less than 10% of the controls.Hyperstable U1snRNA might be a novel approach to express effective antisense RNA in target cells.
Affiliation: Investigative Treatment Division, National Cancer Center Research Institute East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan.Show MeSH
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Mentions: Cell morphology of PANC-1 clones transfected with hyperstable U1snRNA. Parent PANC-1 (A) and transfectants, Pr1A-4 (B), Pr1S (C), Pr3A-4 (D), Pr3A-5 (E), and Pr3S (F) were cultured to confluence and photographed at a magnification of ×200. The r1A and r3A transfected clones are heterogeneous in their cell morphology and contain many multinucleated giant cells.
Affiliation: Investigative Treatment Division, National Cancer Center Research Institute East, 6-5-1, Kashiwanoha, Kashiwa, Chiba 277-8577, Japan.