Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin.
Bottom Line: We found that a lectin, Datura stramonium agglutinin, induced irreversible differentiation in C6 glioma cells.Further, these differentiated human glial tumour cells had long processes and a high content of glial fibrillary acidic protein similar to differentiated C6 glioma cells.Taken together, these observations suggest that Datura stramonium agglutinin may be useful as a new therapy for treating glioma without side effects.
Affiliation: Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.Show MeSH
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Mentions: The malignancy of gliomas is inversely correlated with the content of astrocyte-specific intermediate filament protein (GFAP) (Tascos et al, 1982). Furthermore, mature astrocytes are characterised by the expression of a large amount of GFAP (Tascos et al, 1982; Chiu and Goldman, 1985). GFAP is expressed exclusively in astrocytes and the expression level increases during differentiation. C6 cells were found to produce only a small amount of GFAP due to their malignant properties (Messens and Slegers, 1992). The immunoblot in Figure 3Figure 3
Affiliation: Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.