Limits...
Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin.

Sasaki T, Yamazaki K, Yamori T, Endo T - Br. J. Cancer (2002)

Bottom Line: We found that a lectin, Datura stramonium agglutinin, induced irreversible differentiation in C6 glioma cells.Further, these differentiated human glial tumour cells had long processes and a high content of glial fibrillary acidic protein similar to differentiated C6 glioma cells.Taken together, these observations suggest that Datura stramonium agglutinin may be useful as a new therapy for treating glioma without side effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.

Show MeSH

Related in: MedlinePlus

Immunoblotting with anti-GFAP antibody. Cells cultured in media containing 10% FBS, were collected 24 h after incubation without (lanes 1, 3, 5, 7 and 9) and with DSA (lanes 2, 4, 6, 8 and 10). Lanes 1 and 2, U251; lanes 3 and 4, SNB-75; lanes 5 and 6, SNB-78; lanes 7 and 8, SF-539; lanes 9 and 10, C6. SDS-solubilised fractions obtained from each cell preparation were applied to SDS–PAGE using a 10% acrylamide gel, and the separated proteins were transferred to a PVDF membrane. Lanes from 1 to 8 contained 5 μg protein, and lanes 9 and 10 did 10 μg. The PVDF membrane was stained with anti-GFAP antibody. The migration positions of GFAP and molecular weight markers (kilodalton) are indicated (lane 11).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2376164&req=5

fig3: Immunoblotting with anti-GFAP antibody. Cells cultured in media containing 10% FBS, were collected 24 h after incubation without (lanes 1, 3, 5, 7 and 9) and with DSA (lanes 2, 4, 6, 8 and 10). Lanes 1 and 2, U251; lanes 3 and 4, SNB-75; lanes 5 and 6, SNB-78; lanes 7 and 8, SF-539; lanes 9 and 10, C6. SDS-solubilised fractions obtained from each cell preparation were applied to SDS–PAGE using a 10% acrylamide gel, and the separated proteins were transferred to a PVDF membrane. Lanes from 1 to 8 contained 5 μg protein, and lanes 9 and 10 did 10 μg. The PVDF membrane was stained with anti-GFAP antibody. The migration positions of GFAP and molecular weight markers (kilodalton) are indicated (lane 11).

Mentions: The malignancy of gliomas is inversely correlated with the content of astrocyte-specific intermediate filament protein (GFAP) (Tascos et al, 1982). Furthermore, mature astrocytes are characterised by the expression of a large amount of GFAP (Tascos et al, 1982; Chiu and Goldman, 1985). GFAP is expressed exclusively in astrocytes and the expression level increases during differentiation. C6 cells were found to produce only a small amount of GFAP due to their malignant properties (Messens and Slegers, 1992). The immunoblot in Figure 3Figure 3


Inhibition of proliferation and induction of differentiation of glioma cells with Datura stramonium agglutinin.

Sasaki T, Yamazaki K, Yamori T, Endo T - Br. J. Cancer (2002)

Immunoblotting with anti-GFAP antibody. Cells cultured in media containing 10% FBS, were collected 24 h after incubation without (lanes 1, 3, 5, 7 and 9) and with DSA (lanes 2, 4, 6, 8 and 10). Lanes 1 and 2, U251; lanes 3 and 4, SNB-75; lanes 5 and 6, SNB-78; lanes 7 and 8, SF-539; lanes 9 and 10, C6. SDS-solubilised fractions obtained from each cell preparation were applied to SDS–PAGE using a 10% acrylamide gel, and the separated proteins were transferred to a PVDF membrane. Lanes from 1 to 8 contained 5 μg protein, and lanes 9 and 10 did 10 μg. The PVDF membrane was stained with anti-GFAP antibody. The migration positions of GFAP and molecular weight markers (kilodalton) are indicated (lane 11).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376164&req=5

fig3: Immunoblotting with anti-GFAP antibody. Cells cultured in media containing 10% FBS, were collected 24 h after incubation without (lanes 1, 3, 5, 7 and 9) and with DSA (lanes 2, 4, 6, 8 and 10). Lanes 1 and 2, U251; lanes 3 and 4, SNB-75; lanes 5 and 6, SNB-78; lanes 7 and 8, SF-539; lanes 9 and 10, C6. SDS-solubilised fractions obtained from each cell preparation were applied to SDS–PAGE using a 10% acrylamide gel, and the separated proteins were transferred to a PVDF membrane. Lanes from 1 to 8 contained 5 μg protein, and lanes 9 and 10 did 10 μg. The PVDF membrane was stained with anti-GFAP antibody. The migration positions of GFAP and molecular weight markers (kilodalton) are indicated (lane 11).
Mentions: The malignancy of gliomas is inversely correlated with the content of astrocyte-specific intermediate filament protein (GFAP) (Tascos et al, 1982). Furthermore, mature astrocytes are characterised by the expression of a large amount of GFAP (Tascos et al, 1982; Chiu and Goldman, 1985). GFAP is expressed exclusively in astrocytes and the expression level increases during differentiation. C6 cells were found to produce only a small amount of GFAP due to their malignant properties (Messens and Slegers, 1992). The immunoblot in Figure 3Figure 3

Bottom Line: We found that a lectin, Datura stramonium agglutinin, induced irreversible differentiation in C6 glioma cells.Further, these differentiated human glial tumour cells had long processes and a high content of glial fibrillary acidic protein similar to differentiated C6 glioma cells.Taken together, these observations suggest that Datura stramonium agglutinin may be useful as a new therapy for treating glioma without side effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashi-ku, Tokyo 173-0015, Japan.

Show MeSH
Related in: MedlinePlus