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Delta-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters.

Bermúdez Moretti M, Correa García S, Perotti C, Batlle A, Casas A - Br. J. Cancer (2002)

Bottom Line: The aim of this work was to characterise the delta-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-delta-aminolevulinic acid, to finally improve delta-aminolevulinic acid incorporation in mammalian cells.Our results showed that delta-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport.Specificity assays suggested that the transporter involved in delta-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), FCEN, University of Buenos Aires, CONICET. Ciudad Universitaria, Pabellón II, 2 degrees Piso, 1428, Buenos Aires, Argentina.

ABSTRACT
Delta-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the delta-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-delta-aminolevulinic acid, to finally improve delta-aminolevulinic acid incorporation in mammalian cells. Our results showed that delta-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in delta-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2.

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Dependence of GABA uptake on temperature. GABA uptake was measured at 37°C or 4°C in the absence or the presence of ALA. When uptake was determined in the presence of ALA, cells were preincubated 15 min with 10 mM ALA. Then 14C-GABA was added and radioactivity was measured.
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fig4: Dependence of GABA uptake on temperature. GABA uptake was measured at 37°C or 4°C in the absence or the presence of ALA. When uptake was determined in the presence of ALA, cells were preincubated 15 min with 10 mM ALA. Then 14C-GABA was added and radioactivity was measured.

Mentions: Figure 4Figure 4


Delta-Aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters.

Bermúdez Moretti M, Correa García S, Perotti C, Batlle A, Casas A - Br. J. Cancer (2002)

Dependence of GABA uptake on temperature. GABA uptake was measured at 37°C or 4°C in the absence or the presence of ALA. When uptake was determined in the presence of ALA, cells were preincubated 15 min with 10 mM ALA. Then 14C-GABA was added and radioactivity was measured.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376128&req=5

fig4: Dependence of GABA uptake on temperature. GABA uptake was measured at 37°C or 4°C in the absence or the presence of ALA. When uptake was determined in the presence of ALA, cells were preincubated 15 min with 10 mM ALA. Then 14C-GABA was added and radioactivity was measured.
Mentions: Figure 4Figure 4

Bottom Line: The aim of this work was to characterise the delta-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-delta-aminolevulinic acid, to finally improve delta-aminolevulinic acid incorporation in mammalian cells.Our results showed that delta-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport.Specificity assays suggested that the transporter involved in delta-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), FCEN, University of Buenos Aires, CONICET. Ciudad Universitaria, Pabellón II, 2 degrees Piso, 1428, Buenos Aires, Argentina.

ABSTRACT
Delta-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the delta-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using (14)C-delta-aminolevulinic acid, to finally improve delta-aminolevulinic acid incorporation in mammalian cells. Our results showed that delta-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in delta-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2.

Show MeSH
Related in: MedlinePlus