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Increased expression of beta 2-microglobulin in multidrug-resistant tumour cells.

Scheffer GL, de Jong MC, Monks A, Flens MJ, Hose CD, Izquierdo MA, Shoemaker RH, Scheper RJ - Br. J. Cancer (2002)

Bottom Line: The LMR-12/ beta2-m staining results were confirmed by mRNA microarray data from an independent National Cancer Institute study, as well as by newly obtained reverse transcriptase polymerase chain reaction data.Further analysis of the microarray data showed that beta2-m levels closely reflected levels of major histocompatibility complex class I heavy chains and the transporter associated with antigen processing.Since the ABC transporter associated with antigen processing was previously shown to contribute to multidrug-resistance, it may very well be that the observed LMR-12/ beta2-m levels are secondary to (elevated) levels of the transporter associated with antigen processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands. rj.scheper@vumc.nl

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Staining of frozen sections of tumour samples with LMR-12, W6/32 and control Mab. Upper panel: small intestinal cancer samples (showing only staining in the surrounding tissue). Lower panel: lung cancer samples (showing staining in the tumour cells and in the surrounding tissue). In the two panels a similar staining pattern is observed for both LMR-12/ β2-m and W6/32 HLA class I molecules. Colour development was with 0.02% (w v−1) amino-ethyl-carbazole and 0.02% (v v−1) H2O2.
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fig4: Staining of frozen sections of tumour samples with LMR-12, W6/32 and control Mab. Upper panel: small intestinal cancer samples (showing only staining in the surrounding tissue). Lower panel: lung cancer samples (showing staining in the tumour cells and in the surrounding tissue). In the two panels a similar staining pattern is observed for both LMR-12/ β2-m and W6/32 HLA class I molecules. Colour development was with 0.02% (w v−1) amino-ethyl-carbazole and 0.02% (v v−1) H2O2.

Mentions: In a panel of 34 human tumours of different histogenetic origin, clear LMR-12/ β2-m staining was observed in ∼90% of the tumour samples, while the surrounding normal tissue always stained positive. The staining pattern of LMR-12/ β2-m was closely paralleled by W6/32 staining (Figure 4Figure 4


Increased expression of beta 2-microglobulin in multidrug-resistant tumour cells.

Scheffer GL, de Jong MC, Monks A, Flens MJ, Hose CD, Izquierdo MA, Shoemaker RH, Scheper RJ - Br. J. Cancer (2002)

Staining of frozen sections of tumour samples with LMR-12, W6/32 and control Mab. Upper panel: small intestinal cancer samples (showing only staining in the surrounding tissue). Lower panel: lung cancer samples (showing staining in the tumour cells and in the surrounding tissue). In the two panels a similar staining pattern is observed for both LMR-12/ β2-m and W6/32 HLA class I molecules. Colour development was with 0.02% (w v−1) amino-ethyl-carbazole and 0.02% (v v−1) H2O2.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375440&req=5

fig4: Staining of frozen sections of tumour samples with LMR-12, W6/32 and control Mab. Upper panel: small intestinal cancer samples (showing only staining in the surrounding tissue). Lower panel: lung cancer samples (showing staining in the tumour cells and in the surrounding tissue). In the two panels a similar staining pattern is observed for both LMR-12/ β2-m and W6/32 HLA class I molecules. Colour development was with 0.02% (w v−1) amino-ethyl-carbazole and 0.02% (v v−1) H2O2.
Mentions: In a panel of 34 human tumours of different histogenetic origin, clear LMR-12/ β2-m staining was observed in ∼90% of the tumour samples, while the surrounding normal tissue always stained positive. The staining pattern of LMR-12/ β2-m was closely paralleled by W6/32 staining (Figure 4Figure 4

Bottom Line: The LMR-12/ beta2-m staining results were confirmed by mRNA microarray data from an independent National Cancer Institute study, as well as by newly obtained reverse transcriptase polymerase chain reaction data.Further analysis of the microarray data showed that beta2-m levels closely reflected levels of major histocompatibility complex class I heavy chains and the transporter associated with antigen processing.Since the ABC transporter associated with antigen processing was previously shown to contribute to multidrug-resistance, it may very well be that the observed LMR-12/ beta2-m levels are secondary to (elevated) levels of the transporter associated with antigen processing.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands. rj.scheper@vumc.nl

Show MeSH
Related in: MedlinePlus