Increased expression of beta 2-microglobulin in multidrug-resistant tumour cells.
Bottom Line: The LMR-12/ beta2-m staining results were confirmed by mRNA microarray data from an independent National Cancer Institute study, as well as by newly obtained reverse transcriptase polymerase chain reaction data.Further analysis of the microarray data showed that beta2-m levels closely reflected levels of major histocompatibility complex class I heavy chains and the transporter associated with antigen processing.Since the ABC transporter associated with antigen processing was previously shown to contribute to multidrug-resistance, it may very well be that the observed LMR-12/ beta2-m levels are secondary to (elevated) levels of the transporter associated with antigen processing.
Affiliation: Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands. firstname.lastname@example.orgShow MeSH
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Mentions: To confirm the expression data on β2-m in the cell line panel, the correlation between the LMR-12/ β2-m staining data and microarray data on mRNA levels for β2-m was examined. Ross et al (2000) and Scherf et al (2000) have reported microarray data on approximately 8000 genes in the cell lines of the NCI panel, including β2-m. These data are available via http://dtp.nci.nih.gov. From a total of 45 cell lines, both protein and mRNA data could be compared. The analysis showed a moderate, but highly significant correlation (Tables 1 and 2Table 2Pearson correlation coefficients for HLA-A, HLA-B, β2-m and TAP1 mRNA microarray analyses, and β2-m RT–PCR and LMR-12/β2-m staining results and Figure 2Figure 2
Affiliation: Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands. email@example.com