The prognostic influence of bcl-2 in malignant glioma.
Bottom Line: The bcl-2 gene is one of a complex group of genes which control programmed cell death.Bcl-2 immunostaining was assessed in 159 specimens from these patients, by scoring systems of 0 to 3 for intensity of scoring and proportion of cells staining.The expression of bcl-2 had a significant relationship with survival (univariate Cox model P=0.0302, hazard ratio 0.8, 95% confidence interval 0.65-0.98), with increased staining associated with improved survival.
Affiliation: Northern Centre for Cancer Treatment, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK. email@example.comShow MeSH
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Mentions: Immunostaining was assessed by semiquantitative scoring systems for percentage of cells staining and intensity of staining. Both were scored on scales of 0 to 3. For percentage of cells staining, a score of 0 represented no cells staining, a score of 1 represented less than 25%, a score of 2, 25 to 50%, and a score of 3 over 50% of cells staining. To establish a scale for intensity of staining an initial pilot group of sections was examined to observe the range of staining. A semiquantative scale of 0 to 3 was then used to assess the intensity of staining. See Figure 1Figure 1
Affiliation: Northern Centre for Cancer Treatment, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK. firstname.lastname@example.org