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Overexpression of Betaig-h3 gene downregulates integrin alpha5beta1 and suppresses tumorigenicity in radiation-induced tumorigenic human bronchial epithelial cells.

Zhao YL, Piao CQ, Hei TK - Br. J. Cancer (2002)

Bottom Line: Using an immortalised human bronchial epithelial (BEP2D) cell model, the study here shows that expression of Betaig-h3 gene, which encodes a secreted adhesion molecule induced by transforming growth factor-beta, is markedly decreased in several independently generated, radiation-induced tumour cell lines (TL1-TL5) relative to parental BEP2D cells.Transfection of Betaig-h3 gene into tumour cells resulted in a significant reduction in tumour growth.These data suggest that Betaig-h3 gene is involved in tumour progression by regulating integrin receptor alpha5beta1.

View Article: PubMed Central - PubMed

Affiliation: Center for Radiological Research, College of Physicians and Surgeons of Columbia University, VC 11-218, 630 West 168th Street, New York, NY 10032, USA.

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(A) mRNA and protein levels of Betaig-h3 gene determined by Northern blot and immunoblotting (IB) in normal NHBE, control BEP2D, TL1 and Betaig-h3-transfected tumour cells. (B) In vitro growth rate of parental TL1 and Betaig-h3-transfected tumour cells. Data represent mean±s.d. of eight culture flasks from two independent experiments. (C) Inhibition of tumour growth by Betaig-h3 transfection relative to vector alone and parental TL1 tumour cells. Results are expressed as the mean±s.d. of 8–9 independent tumours.
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fig2: (A) mRNA and protein levels of Betaig-h3 gene determined by Northern blot and immunoblotting (IB) in normal NHBE, control BEP2D, TL1 and Betaig-h3-transfected tumour cells. (B) In vitro growth rate of parental TL1 and Betaig-h3-transfected tumour cells. Data represent mean±s.d. of eight culture flasks from two independent experiments. (C) Inhibition of tumour growth by Betaig-h3 transfection relative to vector alone and parental TL1 tumour cells. Results are expressed as the mean±s.d. of 8–9 independent tumours.

Mentions: To examine the significance of Betaig-h3 downregulation in malignant conversion, we recovered the expression of Betaig-h3 gene in a representative tumour cell line (TL1) with pRc/CMV2-Betaigh3 vector. Two G418-resistant colonies (TL1-clones 18 and TL1-clone 28) that expressed different levels of Betaig-h3 were chosen for further studies. From the Northern and Western blot results (Figure 2AFigure 2


Overexpression of Betaig-h3 gene downregulates integrin alpha5beta1 and suppresses tumorigenicity in radiation-induced tumorigenic human bronchial epithelial cells.

Zhao YL, Piao CQ, Hei TK - Br. J. Cancer (2002)

(A) mRNA and protein levels of Betaig-h3 gene determined by Northern blot and immunoblotting (IB) in normal NHBE, control BEP2D, TL1 and Betaig-h3-transfected tumour cells. (B) In vitro growth rate of parental TL1 and Betaig-h3-transfected tumour cells. Data represent mean±s.d. of eight culture flasks from two independent experiments. (C) Inhibition of tumour growth by Betaig-h3 transfection relative to vector alone and parental TL1 tumour cells. Results are expressed as the mean±s.d. of 8–9 independent tumours.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2375424&req=5

fig2: (A) mRNA and protein levels of Betaig-h3 gene determined by Northern blot and immunoblotting (IB) in normal NHBE, control BEP2D, TL1 and Betaig-h3-transfected tumour cells. (B) In vitro growth rate of parental TL1 and Betaig-h3-transfected tumour cells. Data represent mean±s.d. of eight culture flasks from two independent experiments. (C) Inhibition of tumour growth by Betaig-h3 transfection relative to vector alone and parental TL1 tumour cells. Results are expressed as the mean±s.d. of 8–9 independent tumours.
Mentions: To examine the significance of Betaig-h3 downregulation in malignant conversion, we recovered the expression of Betaig-h3 gene in a representative tumour cell line (TL1) with pRc/CMV2-Betaigh3 vector. Two G418-resistant colonies (TL1-clones 18 and TL1-clone 28) that expressed different levels of Betaig-h3 were chosen for further studies. From the Northern and Western blot results (Figure 2AFigure 2

Bottom Line: Using an immortalised human bronchial epithelial (BEP2D) cell model, the study here shows that expression of Betaig-h3 gene, which encodes a secreted adhesion molecule induced by transforming growth factor-beta, is markedly decreased in several independently generated, radiation-induced tumour cell lines (TL1-TL5) relative to parental BEP2D cells.Transfection of Betaig-h3 gene into tumour cells resulted in a significant reduction in tumour growth.These data suggest that Betaig-h3 gene is involved in tumour progression by regulating integrin receptor alpha5beta1.

View Article: PubMed Central - PubMed

Affiliation: Center for Radiological Research, College of Physicians and Surgeons of Columbia University, VC 11-218, 630 West 168th Street, New York, NY 10032, USA.

Show MeSH
Related in: MedlinePlus