Overexpression of Betaig-h3 gene downregulates integrin alpha5beta1 and suppresses tumorigenicity in radiation-induced tumorigenic human bronchial epithelial cells.
Bottom Line: Using an immortalised human bronchial epithelial (BEP2D) cell model, the study here shows that expression of Betaig-h3 gene, which encodes a secreted adhesion molecule induced by transforming growth factor-beta, is markedly decreased in several independently generated, radiation-induced tumour cell lines (TL1-TL5) relative to parental BEP2D cells.Transfection of Betaig-h3 gene into tumour cells resulted in a significant reduction in tumour growth.These data suggest that Betaig-h3 gene is involved in tumour progression by regulating integrin receptor alpha5beta1.
Affiliation: Center for Radiological Research, College of Physicians and Surgeons of Columbia University, VC 11-218, 630 West 168th Street, New York, NY 10032, USA.Show MeSH
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Mentions: To examine the significance of Betaig-h3 downregulation in malignant conversion, we recovered the expression of Betaig-h3 gene in a representative tumour cell line (TL1) with pRc/CMV2-Betaigh3 vector. Two G418-resistant colonies (TL1-clones 18 and TL1-clone 28) that expressed different levels of Betaig-h3 were chosen for further studies. From the Northern and Western blot results (Figure 2AFigure 2
Affiliation: Center for Radiological Research, College of Physicians and Surgeons of Columbia University, VC 11-218, 630 West 168th Street, New York, NY 10032, USA.