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DNA methylation and histone deacetylation associated with silencing DAP kinase gene expression in colorectal and gastric cancers.

Satoh A, Toyota M, Itoh F, Kikuchi T, Obata T, Sasaki Y, Suzuki H, Yawata A, Kusano M, Fujita M, Hosokawa M, Yanagihara K, Tokino T, Imai K - Br. J. Cancer (2002)

Bottom Line: Death-associated protein kinase is a positive regulator of programmed cell death induced by interferon gamma.Death-associated protein kinase expression was diminished in four cell lines that showed dense methylation of the 5' CpG island, and treatment with 5-aza-2'-deoxycitidine, a methyltransferase inhibitor, restored gene expression.Thus, aberrant DNA methylation and histone deacetylation of the 5' CpG island, but not the edge of the CpG island, appears to play a key role in silencing death-associated protein kinase expression in gastrointestinal malignancies.

View Article: PubMed Central - PubMed

Affiliation: First Department of Internal Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060-8543, Japan.

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Summary of methylation levels in colorectal (CRC) and gastric cancer (GC) cell lines, which were divided into five groups based on the methylation density of each region. The percentages were calculated by densitometry and are indicted by the boxes. Group 1, CRC: HT29, GC: HSC41, MKN45, NUGC3 and NUGC4 cells; group 2, CRC: Caco2, SW48, DLD-1, LoVo, GC: HSC39, HSC40, HSC42, MKN7, MKN74, AZ521, JRST and NUGC2 cells; group 3: CRC: HCT116, GC: HSC43 and SH101; group 4: CRC: RKO, SW480, GC: MKN28, HSC44 and HSC45; group 5: CRC: Colo320.
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fig4: Summary of methylation levels in colorectal (CRC) and gastric cancer (GC) cell lines, which were divided into five groups based on the methylation density of each region. The percentages were calculated by densitometry and are indicted by the boxes. Group 1, CRC: HT29, GC: HSC41, MKN45, NUGC3 and NUGC4 cells; group 2, CRC: Caco2, SW48, DLD-1, LoVo, GC: HSC39, HSC40, HSC42, MKN7, MKN74, AZ521, JRST and NUGC2 cells; group 3: CRC: HCT116, GC: HSC43 and SH101; group 4: CRC: RKO, SW480, GC: MKN28, HSC44 and HSC45; group 5: CRC: Colo320.

Mentions: Summary of methylation level of each region and expression of DAPK is shown in Figure 4Figure 4


DNA methylation and histone deacetylation associated with silencing DAP kinase gene expression in colorectal and gastric cancers.

Satoh A, Toyota M, Itoh F, Kikuchi T, Obata T, Sasaki Y, Suzuki H, Yawata A, Kusano M, Fujita M, Hosokawa M, Yanagihara K, Tokino T, Imai K - Br. J. Cancer (2002)

Summary of methylation levels in colorectal (CRC) and gastric cancer (GC) cell lines, which were divided into five groups based on the methylation density of each region. The percentages were calculated by densitometry and are indicted by the boxes. Group 1, CRC: HT29, GC: HSC41, MKN45, NUGC3 and NUGC4 cells; group 2, CRC: Caco2, SW48, DLD-1, LoVo, GC: HSC39, HSC40, HSC42, MKN7, MKN74, AZ521, JRST and NUGC2 cells; group 3: CRC: HCT116, GC: HSC43 and SH101; group 4: CRC: RKO, SW480, GC: MKN28, HSC44 and HSC45; group 5: CRC: Colo320.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375414&req=5

fig4: Summary of methylation levels in colorectal (CRC) and gastric cancer (GC) cell lines, which were divided into five groups based on the methylation density of each region. The percentages were calculated by densitometry and are indicted by the boxes. Group 1, CRC: HT29, GC: HSC41, MKN45, NUGC3 and NUGC4 cells; group 2, CRC: Caco2, SW48, DLD-1, LoVo, GC: HSC39, HSC40, HSC42, MKN7, MKN74, AZ521, JRST and NUGC2 cells; group 3: CRC: HCT116, GC: HSC43 and SH101; group 4: CRC: RKO, SW480, GC: MKN28, HSC44 and HSC45; group 5: CRC: Colo320.
Mentions: Summary of methylation level of each region and expression of DAPK is shown in Figure 4Figure 4

Bottom Line: Death-associated protein kinase is a positive regulator of programmed cell death induced by interferon gamma.Death-associated protein kinase expression was diminished in four cell lines that showed dense methylation of the 5' CpG island, and treatment with 5-aza-2'-deoxycitidine, a methyltransferase inhibitor, restored gene expression.Thus, aberrant DNA methylation and histone deacetylation of the 5' CpG island, but not the edge of the CpG island, appears to play a key role in silencing death-associated protein kinase expression in gastrointestinal malignancies.

View Article: PubMed Central - PubMed

Affiliation: First Department of Internal Medicine, Sapporo Medical University, S-1, W-16, Chuo-ku, Sapporo 060-8543, Japan.

Show MeSH
Related in: MedlinePlus