Serological identification and expression analysis of gastric cancer-associated genes.
Bottom Line: In the present study we applied this technique to identify immunogenic proteins for gastric cancer that resulted in isolation of 14 distinct serum-reactive antigens.An unusual DNA polymorphism--a three-nucleotide deletion was found in NUCB2 cDNA but its mRNA level was consistently decreased in gastric tumours compared with that in the adjacent non-cancerous tissues.This study has revealed several new gastric cancer candidate genes; additional studies are required to gain a deeper insight into their role in the tumorigenesis and their potential as therapeutic targets.
Affiliation: Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites St, LV-1067, Riga, Latvia. email@example.comShow MeSH
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Mentions: In order to evaluate whether expression levels of genes encoding SEREX-antigens are altered in cancer tissues, we compared the relative mRNA levels in cancerous and paired non-cancerous tissues by semi-quantitative RT–PCR. Expression levels of nine genes isolated from gastric cancer cDNA library (Ga19, Ga27, Ga34, Ga50, Ga55, Zg2, Zg4, Zg14, and Zg15) were analysed in paired tissue samples from 20 gastric cancer patients. Relative mRNA levels of target genes were normalised to GAPDH and β-actin, and Tumour/Normal (T/N) ratios were calculated. Each T/N value represents the mean value of two independent experiments usually differing in less than 20%. T/N values 2 or ⩽0.5 were considered to represent significant difference. Histone H4 cDNA was amplified as a marker for the rate of proliferating cells in the tissue sample. Relative overexpression of three genes in various numbers of tumour specimens was observed. An example of the RT–PCR results is shown in Figure 2Figure 2
Affiliation: Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites St, LV-1067, Riga, Latvia. firstname.lastname@example.org