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Serological identification and expression analysis of gastric cancer-associated genes.

Linē A, Stengrēvics A, Slucka Z, Li G, Jankevics E, Rees RC - Br. J. Cancer (2002)

Bottom Line: In the present study we applied this technique to identify immunogenic proteins for gastric cancer that resulted in isolation of 14 distinct serum-reactive antigens.An unusual DNA polymorphism--a three-nucleotide deletion was found in NUCB2 cDNA but its mRNA level was consistently decreased in gastric tumours compared with that in the adjacent non-cancerous tissues.This study has revealed several new gastric cancer candidate genes; additional studies are required to gain a deeper insight into their role in the tumorigenesis and their potential as therapeutic targets.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites St, LV-1067, Riga, Latvia. aija@biomed.lu.lv

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Comparison of Ga19, Zg4 and Zg15 mRNA levels in three paired gastric cancer (T) and adjacent epithelium (N) samples by semi-quantitative RT–PCR. The same primers as shown in Figure 1 were used for amplification of Ga19 and Zg4. Primers used for Zg15 were: forward primer 5′-TCTGGAACTATCTTTACCCAG-3′, reverse 5′-GTTTGACGTGCTGAGCAAGC-3′. GAPDH and β-actin are used as internal standards. Histone H4 is used as a marker for the rate of cell proliferation.
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fig2: Comparison of Ga19, Zg4 and Zg15 mRNA levels in three paired gastric cancer (T) and adjacent epithelium (N) samples by semi-quantitative RT–PCR. The same primers as shown in Figure 1 were used for amplification of Ga19 and Zg4. Primers used for Zg15 were: forward primer 5′-TCTGGAACTATCTTTACCCAG-3′, reverse 5′-GTTTGACGTGCTGAGCAAGC-3′. GAPDH and β-actin are used as internal standards. Histone H4 is used as a marker for the rate of cell proliferation.

Mentions: In order to evaluate whether expression levels of genes encoding SEREX-antigens are altered in cancer tissues, we compared the relative mRNA levels in cancerous and paired non-cancerous tissues by semi-quantitative RT–PCR. Expression levels of nine genes isolated from gastric cancer cDNA library (Ga19, Ga27, Ga34, Ga50, Ga55, Zg2, Zg4, Zg14, and Zg15) were analysed in paired tissue samples from 20 gastric cancer patients. Relative mRNA levels of target genes were normalised to GAPDH and β-actin, and Tumour/Normal (T/N) ratios were calculated. Each T/N value represents the mean value of two independent experiments usually differing in less than 20%. T/N values 2 or ⩽0.5 were considered to represent significant difference. Histone H4 cDNA was amplified as a marker for the rate of proliferating cells in the tissue sample. Relative overexpression of three genes in various numbers of tumour specimens was observed. An example of the RT–PCR results is shown in Figure 2Figure 2


Serological identification and expression analysis of gastric cancer-associated genes.

Linē A, Stengrēvics A, Slucka Z, Li G, Jankevics E, Rees RC - Br. J. Cancer (2002)

Comparison of Ga19, Zg4 and Zg15 mRNA levels in three paired gastric cancer (T) and adjacent epithelium (N) samples by semi-quantitative RT–PCR. The same primers as shown in Figure 1 were used for amplification of Ga19 and Zg4. Primers used for Zg15 were: forward primer 5′-TCTGGAACTATCTTTACCCAG-3′, reverse 5′-GTTTGACGTGCTGAGCAAGC-3′. GAPDH and β-actin are used as internal standards. Histone H4 is used as a marker for the rate of cell proliferation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375403&req=5

fig2: Comparison of Ga19, Zg4 and Zg15 mRNA levels in three paired gastric cancer (T) and adjacent epithelium (N) samples by semi-quantitative RT–PCR. The same primers as shown in Figure 1 were used for amplification of Ga19 and Zg4. Primers used for Zg15 were: forward primer 5′-TCTGGAACTATCTTTACCCAG-3′, reverse 5′-GTTTGACGTGCTGAGCAAGC-3′. GAPDH and β-actin are used as internal standards. Histone H4 is used as a marker for the rate of cell proliferation.
Mentions: In order to evaluate whether expression levels of genes encoding SEREX-antigens are altered in cancer tissues, we compared the relative mRNA levels in cancerous and paired non-cancerous tissues by semi-quantitative RT–PCR. Expression levels of nine genes isolated from gastric cancer cDNA library (Ga19, Ga27, Ga34, Ga50, Ga55, Zg2, Zg4, Zg14, and Zg15) were analysed in paired tissue samples from 20 gastric cancer patients. Relative mRNA levels of target genes were normalised to GAPDH and β-actin, and Tumour/Normal (T/N) ratios were calculated. Each T/N value represents the mean value of two independent experiments usually differing in less than 20%. T/N values 2 or ⩽0.5 were considered to represent significant difference. Histone H4 cDNA was amplified as a marker for the rate of proliferating cells in the tissue sample. Relative overexpression of three genes in various numbers of tumour specimens was observed. An example of the RT–PCR results is shown in Figure 2Figure 2

Bottom Line: In the present study we applied this technique to identify immunogenic proteins for gastric cancer that resulted in isolation of 14 distinct serum-reactive antigens.An unusual DNA polymorphism--a three-nucleotide deletion was found in NUCB2 cDNA but its mRNA level was consistently decreased in gastric tumours compared with that in the adjacent non-cancerous tissues.This study has revealed several new gastric cancer candidate genes; additional studies are required to gain a deeper insight into their role in the tumorigenesis and their potential as therapeutic targets.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites St, LV-1067, Riga, Latvia. aija@biomed.lu.lv

Show MeSH
Related in: MedlinePlus