Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.
Bottom Line: Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin.Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene.These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy.
Affiliation: Department of Surgery II, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama, 359-8513 Japan.Show MeSH
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Mentions: Human microvascular endothelial cells were able to grow in medium in the presence of 10% FBS without addition of a specific growth factor. When VEGF165, FGF and HGF were added to the culture medium, the growth rate of HMVEC increased to 1.2–1.7-fold when compared to the control. Horizontal lines in Figure 3A,BFigure 3
Affiliation: Department of Surgery II, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama, 359-8513 Japan.