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Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.

Ono K, Ishihara M, Ishikawa K, Ozeki Y, Deguchi H, Sato M, Hashimoto H, Saito Y, Yura H, Kurita A, Maehara T - Br. J. Cancer (2002)

Bottom Line: Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin.Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene.These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery II, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama, 359-8513 Japan.

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Effect of NAC-HCPS on growth factor-stimulated HMVEC growth. (A) VEGF165, (B) FGF-2, (C) HGF. Cell growth incubated with a growth factor in the absence of heparinoid was defined as 100% growth, and the data were calculated as a percentage. The horizontal line in each panel represents the level of cell growth obtained in the absence of both growth factor and heparinoid. The results represent the mean±s.e. in triplicate.
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fig3: Effect of NAC-HCPS on growth factor-stimulated HMVEC growth. (A) VEGF165, (B) FGF-2, (C) HGF. Cell growth incubated with a growth factor in the absence of heparinoid was defined as 100% growth, and the data were calculated as a percentage. The horizontal line in each panel represents the level of cell growth obtained in the absence of both growth factor and heparinoid. The results represent the mean±s.e. in triplicate.

Mentions: Human microvascular endothelial cells were able to grow in medium in the presence of 10% FBS without addition of a specific growth factor. When VEGF165, FGF and HGF were added to the culture medium, the growth rate of HMVEC increased to 1.2–1.7-fold when compared to the control. Horizontal lines in Figure 3A,BFigure 3


Periodate-treated, non-anticoagulant heparin-carrying polystyrene (NAC-HCPS) affects angiogenesis and inhibits subcutaneous induced tumour growth and metastasis to the lung.

Ono K, Ishihara M, Ishikawa K, Ozeki Y, Deguchi H, Sato M, Hashimoto H, Saito Y, Yura H, Kurita A, Maehara T - Br. J. Cancer (2002)

Effect of NAC-HCPS on growth factor-stimulated HMVEC growth. (A) VEGF165, (B) FGF-2, (C) HGF. Cell growth incubated with a growth factor in the absence of heparinoid was defined as 100% growth, and the data were calculated as a percentage. The horizontal line in each panel represents the level of cell growth obtained in the absence of both growth factor and heparinoid. The results represent the mean±s.e. in triplicate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375397&req=5

fig3: Effect of NAC-HCPS on growth factor-stimulated HMVEC growth. (A) VEGF165, (B) FGF-2, (C) HGF. Cell growth incubated with a growth factor in the absence of heparinoid was defined as 100% growth, and the data were calculated as a percentage. The horizontal line in each panel represents the level of cell growth obtained in the absence of both growth factor and heparinoid. The results represent the mean±s.e. in triplicate.
Mentions: Human microvascular endothelial cells were able to grow in medium in the presence of 10% FBS without addition of a specific growth factor. When VEGF165, FGF and HGF were added to the culture medium, the growth rate of HMVEC increased to 1.2–1.7-fold when compared to the control. Horizontal lines in Figure 3A,BFigure 3

Bottom Line: Periodate-treated, non-anticoagulant heparin-carrying polystyrene consists of about ten periodate-oxidized, alkaline-degraded low molecular weight-heparin chains linked to a polystyrene core and has a markedly lower anti-coagulant activity than heparin.Non-anticoagulant heparin-carrying polystyrene has a higher activity to inhibit vascular endothelial growth factor-165-, fibroblast growth factor-2- or hepatocyte growth factor-induced human microvascular endothelial cell growth than heparin, ten periodate-oxidized-heparin and ten periodate-oxidized-low molecular weight-heparin, which is probably due to the heparin-clustering effect of non-anticoagulant heparin-carrying polystyrene.These results suggest that non-anticoagulant heparin-carrying polystyrene has an inhibitory activity on angiogenesis and tumour invasion and may be very useful in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery II, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama, 359-8513 Japan.

Show MeSH
Related in: MedlinePlus