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CD10 is a marker for cycling cells with propensity to apoptosis in childhood ALL.

Cutrona G, Tasso P, Dono M, Roncella S, Ulivi M, Carpaneto EM, Fontana V, Comis M, Morabito F, Spinelli M, Frascella E, Boffa LC, Basso G, Pistoia V, Ferrarini M - Br. J. Cancer (2002)

Bottom Line: CD10 constitutes a favourable prognostic marker for childhood acute lymphoblastic leukaemia.Twenty-eight cases of childhood acute lymphoblastic leukaemia (mean age of 6.8 years) were subdivided into two groups according to high (17 cases, 93.2+/-4.5%, MRFI 211+/-82 CD10-positive cells) or low (11 cases, 11.5+/-6.2%, MRFI 10+/-7 CD10-negative cells) expression of CD10.Moreover, exposure of CD10-positive acute lymphoblastic leukaemia B cells to a peptide nucleic acid anti-gene specific for the second exon of c-myc caused inhibition of c-myc expression and reduced cell cycling and apoptotic abilities as well as decreased CD10 expression.

View Article: PubMed Central - PubMed

Affiliation: Servizi di Immunologia Clinica, Istituto Nazionale per la Ricerca sul Cancro, IST, Genoa, Italy, and Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, Genoa, Italy. giovanna.cutrona@istge.it

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Inhibition of c-myc expression by PNA-mycwt-NLS. (A) The indicated CD10-positive ALL were incubated with PNA-mycwt-NLS, PNA-mycmut-NLS or medium for 24 h and analysed for Myc expression by flow cytometry. (B and C) Cells from one representative CD10-positive ALL sample (#661) were cultured for 24 h and analysed for c-myc expression by RT–PCR (B) and Western blot (C). (C) Cells from one CD10-positive ALL case (#657) were treated with the indicated reagents for 24 h, pulsed with BrdUrd for 1 h and analysed by flow cytometry to determine the fraction of the cells in the S phase of the cell cycle.
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fig6: Inhibition of c-myc expression by PNA-mycwt-NLS. (A) The indicated CD10-positive ALL were incubated with PNA-mycwt-NLS, PNA-mycmut-NLS or medium for 24 h and analysed for Myc expression by flow cytometry. (B and C) Cells from one representative CD10-positive ALL sample (#661) were cultured for 24 h and analysed for c-myc expression by RT–PCR (B) and Western blot (C). (C) Cells from one CD10-positive ALL case (#657) were treated with the indicated reagents for 24 h, pulsed with BrdUrd for 1 h and analysed by flow cytometry to determine the fraction of the cells in the S phase of the cell cycle.

Mentions: Cells from CD10-positive ALL were incubated with medium, 10 μM PNA-mycwt-NLS, or PNA-mycmut-NLS for 24 h. The cultured cells were harvested and checked for c-myc expression by flow cytometry (Figure 6AFigure 6


CD10 is a marker for cycling cells with propensity to apoptosis in childhood ALL.

Cutrona G, Tasso P, Dono M, Roncella S, Ulivi M, Carpaneto EM, Fontana V, Comis M, Morabito F, Spinelli M, Frascella E, Boffa LC, Basso G, Pistoia V, Ferrarini M - Br. J. Cancer (2002)

Inhibition of c-myc expression by PNA-mycwt-NLS. (A) The indicated CD10-positive ALL were incubated with PNA-mycwt-NLS, PNA-mycmut-NLS or medium for 24 h and analysed for Myc expression by flow cytometry. (B and C) Cells from one representative CD10-positive ALL sample (#661) were cultured for 24 h and analysed for c-myc expression by RT–PCR (B) and Western blot (C). (C) Cells from one CD10-positive ALL case (#657) were treated with the indicated reagents for 24 h, pulsed with BrdUrd for 1 h and analysed by flow cytometry to determine the fraction of the cells in the S phase of the cell cycle.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375395&req=5

fig6: Inhibition of c-myc expression by PNA-mycwt-NLS. (A) The indicated CD10-positive ALL were incubated with PNA-mycwt-NLS, PNA-mycmut-NLS or medium for 24 h and analysed for Myc expression by flow cytometry. (B and C) Cells from one representative CD10-positive ALL sample (#661) were cultured for 24 h and analysed for c-myc expression by RT–PCR (B) and Western blot (C). (C) Cells from one CD10-positive ALL case (#657) were treated with the indicated reagents for 24 h, pulsed with BrdUrd for 1 h and analysed by flow cytometry to determine the fraction of the cells in the S phase of the cell cycle.
Mentions: Cells from CD10-positive ALL were incubated with medium, 10 μM PNA-mycwt-NLS, or PNA-mycmut-NLS for 24 h. The cultured cells were harvested and checked for c-myc expression by flow cytometry (Figure 6AFigure 6

Bottom Line: CD10 constitutes a favourable prognostic marker for childhood acute lymphoblastic leukaemia.Twenty-eight cases of childhood acute lymphoblastic leukaemia (mean age of 6.8 years) were subdivided into two groups according to high (17 cases, 93.2+/-4.5%, MRFI 211+/-82 CD10-positive cells) or low (11 cases, 11.5+/-6.2%, MRFI 10+/-7 CD10-negative cells) expression of CD10.Moreover, exposure of CD10-positive acute lymphoblastic leukaemia B cells to a peptide nucleic acid anti-gene specific for the second exon of c-myc caused inhibition of c-myc expression and reduced cell cycling and apoptotic abilities as well as decreased CD10 expression.

View Article: PubMed Central - PubMed

Affiliation: Servizi di Immunologia Clinica, Istituto Nazionale per la Ricerca sul Cancro, IST, Genoa, Italy, and Dipartimento di Oncologia, Biologia e Genetica, Università di Genova, Genoa, Italy. giovanna.cutrona@istge.it

Show MeSH
Related in: MedlinePlus