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Cell cycle phase perturbations and apoptosis in tumour cells induced by aplidine.

Erba E, Bassano L, Di Liberti G, Muradore I, Chiorino G, Ubezio P, Vignati S, Codegoni A, Desiderio MA, Faircloth G, Jimeno J, D'Incalci M - Br. J. Cancer (2002)

Bottom Line: The drug-induced cell cycle perturbations and subsequent cell death do not appear to be related to macromolecular synthesis (protein, RNA, DNA) since the effects occur at concentrations (e.g. 10 nM) in which macromolecule synthesis was not markedly affected.This finding has questionable relevance since addition of putrescine did not significantly reduce the cell cycle perturbations or the cytotoxicity of Aplidine.The cell cycle perturbations caused by Aplidine were also not due to an effect on the cyclin-dependent kinases.

View Article: PubMed Central - PubMed

Affiliation: Cancer Pharmacology Laboratory, Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, via Eritrea 62, 20157 Milan, Italy. erba@marionegri.it

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(A–C) Percentages of cells in G2M (A) and G1 (C) cell cycle phases. The black columns indicate the percentage of blocked cells in G2M or G1, as resulting from simulation of experimental cell cycle percentages and absolute cell counts. (B–D) Percentage of cell cohort, from the overall initial population, which have died during G2M (B) or G1 (D) block and evaluated at different time intervals after drug-washout (simulation results).
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fig3: (A–C) Percentages of cells in G2M (A) and G1 (C) cell cycle phases. The black columns indicate the percentage of blocked cells in G2M or G1, as resulting from simulation of experimental cell cycle percentages and absolute cell counts. (B–D) Percentage of cell cohort, from the overall initial population, which have died during G2M (B) or G1 (D) block and evaluated at different time intervals after drug-washout (simulation results).

Mentions: We were interested in evaluating the drug's effects on G1, S and G2M phases separately to distinguish blocking or delaying effects from killing ones. Figure 3Figure 3


Cell cycle phase perturbations and apoptosis in tumour cells induced by aplidine.

Erba E, Bassano L, Di Liberti G, Muradore I, Chiorino G, Ubezio P, Vignati S, Codegoni A, Desiderio MA, Faircloth G, Jimeno J, D'Incalci M - Br. J. Cancer (2002)

(A–C) Percentages of cells in G2M (A) and G1 (C) cell cycle phases. The black columns indicate the percentage of blocked cells in G2M or G1, as resulting from simulation of experimental cell cycle percentages and absolute cell counts. (B–D) Percentage of cell cohort, from the overall initial population, which have died during G2M (B) or G1 (D) block and evaluated at different time intervals after drug-washout (simulation results).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375382&req=5

fig3: (A–C) Percentages of cells in G2M (A) and G1 (C) cell cycle phases. The black columns indicate the percentage of blocked cells in G2M or G1, as resulting from simulation of experimental cell cycle percentages and absolute cell counts. (B–D) Percentage of cell cohort, from the overall initial population, which have died during G2M (B) or G1 (D) block and evaluated at different time intervals after drug-washout (simulation results).
Mentions: We were interested in evaluating the drug's effects on G1, S and G2M phases separately to distinguish blocking or delaying effects from killing ones. Figure 3Figure 3

Bottom Line: The drug-induced cell cycle perturbations and subsequent cell death do not appear to be related to macromolecular synthesis (protein, RNA, DNA) since the effects occur at concentrations (e.g. 10 nM) in which macromolecule synthesis was not markedly affected.This finding has questionable relevance since addition of putrescine did not significantly reduce the cell cycle perturbations or the cytotoxicity of Aplidine.The cell cycle perturbations caused by Aplidine were also not due to an effect on the cyclin-dependent kinases.

View Article: PubMed Central - PubMed

Affiliation: Cancer Pharmacology Laboratory, Department of Oncology, Istituto di Ricerche Farmacologiche Mario Negri, via Eritrea 62, 20157 Milan, Italy. erba@marionegri.it

Show MeSH
Related in: MedlinePlus