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Effect of non-steroidal anti-inflammatory drugs on colon carcinoma Caco-2 cell responsiveness to topoisomerase inhibitor drugs.

Ricchi P, Di Matola T, Ruggiero G, Zanzi D, Apicella A, di Palma A, Pensabene M, Pignata S, Zarrilli R, Acquaviva AM - Br. J. Cancer (2002)

Bottom Line: In the present study, we have evaluated the effects of aspirin and NS-398 non-steroidal anti-inflammatory drugs on sensitivity of Caco-2 cells to irinotecan (CPT 11) and etoposide (Vp-16) topoisomerase poisons.Furthermore, aspirin treatment is associated with an increase in bcl-2 expression, which persists in the presence of the anticancer drugs.Our data indicate that aspirin, but not NS-398, determines a cell cycle arrest associated with death suppression.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Istituto di Endocrinologia ed Oncologia Sperimentale G. Salvatore del Consiglio Nazionale delle Ricerche, Università Federico II, Via S. Pansini 5, 80131 Naples, Italy.

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Effect of aspirin and NS-398 co-treatment on 17 μM Vp-16- and 10 μM CPT 11- induced apoptosis of Caco-2 cells. The percentage of viable and apoptotic cells were calculated as reported in Figure 2. Apoptotic bars are the sum of percentage of cells at early and late stages of apoptosis. Data are expressed as mean±s.d. Data points represent the mean of triplicate experiments.
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fig3: Effect of aspirin and NS-398 co-treatment on 17 μM Vp-16- and 10 μM CPT 11- induced apoptosis of Caco-2 cells. The percentage of viable and apoptotic cells were calculated as reported in Figure 2. Apoptotic bars are the sum of percentage of cells at early and late stages of apoptosis. Data are expressed as mean±s.d. Data points represent the mean of triplicate experiments.

Mentions: Effect of aspirin co-treatment on annexin V-FITC/PI staining of Caco-2 cells. Cells at day 3 were incubated with aspirin for 24 h, then anticancer drugs were added in presence of aspirin for 48 h. Four distinct phenotypes become distinguishable: (i) viable cells (lower left quadrant); (ii) cells at early stage of apoptosis (upper left quadrant); (iii) cells at late stage of apoptosis (upper right quadrant); (iiii) necrotic cells (lower right quadrant). (A) untreated cells; (B) cells treated with 2 mM Aspirin; (C) cells treated with 5 mM Aspirin; (D) cell treated with 17 μM Vp-16; (E) cells co-treated with 2 mM aspirin and 17 μM Vp-16; (F) cells co-treated with 5 mM aspirin and 17 μM Vp-16. Data represent one of three similar experiments.


Effect of non-steroidal anti-inflammatory drugs on colon carcinoma Caco-2 cell responsiveness to topoisomerase inhibitor drugs.

Ricchi P, Di Matola T, Ruggiero G, Zanzi D, Apicella A, di Palma A, Pensabene M, Pignata S, Zarrilli R, Acquaviva AM - Br. J. Cancer (2002)

Effect of aspirin and NS-398 co-treatment on 17 μM Vp-16- and 10 μM CPT 11- induced apoptosis of Caco-2 cells. The percentage of viable and apoptotic cells were calculated as reported in Figure 2. Apoptotic bars are the sum of percentage of cells at early and late stages of apoptosis. Data are expressed as mean±s.d. Data points represent the mean of triplicate experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375372&req=5

fig3: Effect of aspirin and NS-398 co-treatment on 17 μM Vp-16- and 10 μM CPT 11- induced apoptosis of Caco-2 cells. The percentage of viable and apoptotic cells were calculated as reported in Figure 2. Apoptotic bars are the sum of percentage of cells at early and late stages of apoptosis. Data are expressed as mean±s.d. Data points represent the mean of triplicate experiments.
Mentions: Effect of aspirin co-treatment on annexin V-FITC/PI staining of Caco-2 cells. Cells at day 3 were incubated with aspirin for 24 h, then anticancer drugs were added in presence of aspirin for 48 h. Four distinct phenotypes become distinguishable: (i) viable cells (lower left quadrant); (ii) cells at early stage of apoptosis (upper left quadrant); (iii) cells at late stage of apoptosis (upper right quadrant); (iiii) necrotic cells (lower right quadrant). (A) untreated cells; (B) cells treated with 2 mM Aspirin; (C) cells treated with 5 mM Aspirin; (D) cell treated with 17 μM Vp-16; (E) cells co-treated with 2 mM aspirin and 17 μM Vp-16; (F) cells co-treated with 5 mM aspirin and 17 μM Vp-16. Data represent one of three similar experiments.

Bottom Line: In the present study, we have evaluated the effects of aspirin and NS-398 non-steroidal anti-inflammatory drugs on sensitivity of Caco-2 cells to irinotecan (CPT 11) and etoposide (Vp-16) topoisomerase poisons.Furthermore, aspirin treatment is associated with an increase in bcl-2 expression, which persists in the presence of the anticancer drugs.Our data indicate that aspirin, but not NS-398, determines a cell cycle arrest associated with death suppression.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Istituto di Endocrinologia ed Oncologia Sperimentale G. Salvatore del Consiglio Nazionale delle Ricerche, Università Federico II, Via S. Pansini 5, 80131 Naples, Italy.

Show MeSH
Related in: MedlinePlus