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Cirrhotic livers reveal genetic changes in the MDM2-P14ARF system of cell cycle regulators.

Schlott T, Scharf JG, Gorzel C, Middel P, Spring H - Br. J. Cancer (2002)

Bottom Line: The P14ARF gene amplified from other specimens did not carry mutations.In summary, this study gives first evidence for different types of MDM2 and P14ARF alterations in cirrhotic livers.We suggest that the changes impair the regulatory MDM2-P14ARF system, thus possibly favouring regenerative proliferation and transformation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytopathology, Georg-August-University, Robert-Koch-Strasse 40, D-37075 Goettingen, Germany. tschlott@med.uni-goettingen.de

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Related in: MedlinePlus

Sequence analysis of MDM2 mRNA splicing variants performed in the automatic DNA analyser. The electropherograms indicate two RNA transcripts differing from subtype a-e published by Sigalas et al, 1996. mRNA (A) and (B) reveal sequence deletions between exon 5 and exon 12. Note the overlapping black and blue peak in sequence B (,,N‘‘) which may indicate heterozygous alteration (G↔C). However, reverse sequencing of this stretch resulted in normal MDM2 sequence (data not shown).
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fig3: Sequence analysis of MDM2 mRNA splicing variants performed in the automatic DNA analyser. The electropherograms indicate two RNA transcripts differing from subtype a-e published by Sigalas et al, 1996. mRNA (A) and (B) reveal sequence deletions between exon 5 and exon 12. Note the overlapping black and blue peak in sequence B (,,N‘‘) which may indicate heterozygous alteration (G↔C). However, reverse sequencing of this stretch resulted in normal MDM2 sequence (data not shown).

Mentions: Detection of MDM2 mRNA splicing variants in alcohol-induced cirrhoses (four samples are shown exemplarily in lane C-F; B contains positive control) and in five autoimmune disease-induced liver cirrhoses (lanes G–K). The samples were HCV negative. Lane A shows molecular weight standard. PCR fragments were separated on 3% agarose gel. The alcohol- and autoimmune disorder-induced cirrhoses express a spectrum of aberrant transcripts. Note that all tissues express full-length MDM2 RNA of 1536 bp except samples H, I, K which only contains splicing variants of MDM2 mRNA. Lanes A, B and E contain faint bands indicating low amounts of normal MDM2 mRNA.


Cirrhotic livers reveal genetic changes in the MDM2-P14ARF system of cell cycle regulators.

Schlott T, Scharf JG, Gorzel C, Middel P, Spring H - Br. J. Cancer (2002)

Sequence analysis of MDM2 mRNA splicing variants performed in the automatic DNA analyser. The electropherograms indicate two RNA transcripts differing from subtype a-e published by Sigalas et al, 1996. mRNA (A) and (B) reveal sequence deletions between exon 5 and exon 12. Note the overlapping black and blue peak in sequence B (,,N‘‘) which may indicate heterozygous alteration (G↔C). However, reverse sequencing of this stretch resulted in normal MDM2 sequence (data not shown).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375354&req=5

fig3: Sequence analysis of MDM2 mRNA splicing variants performed in the automatic DNA analyser. The electropherograms indicate two RNA transcripts differing from subtype a-e published by Sigalas et al, 1996. mRNA (A) and (B) reveal sequence deletions between exon 5 and exon 12. Note the overlapping black and blue peak in sequence B (,,N‘‘) which may indicate heterozygous alteration (G↔C). However, reverse sequencing of this stretch resulted in normal MDM2 sequence (data not shown).
Mentions: Detection of MDM2 mRNA splicing variants in alcohol-induced cirrhoses (four samples are shown exemplarily in lane C-F; B contains positive control) and in five autoimmune disease-induced liver cirrhoses (lanes G–K). The samples were HCV negative. Lane A shows molecular weight standard. PCR fragments were separated on 3% agarose gel. The alcohol- and autoimmune disorder-induced cirrhoses express a spectrum of aberrant transcripts. Note that all tissues express full-length MDM2 RNA of 1536 bp except samples H, I, K which only contains splicing variants of MDM2 mRNA. Lanes A, B and E contain faint bands indicating low amounts of normal MDM2 mRNA.

Bottom Line: The P14ARF gene amplified from other specimens did not carry mutations.In summary, this study gives first evidence for different types of MDM2 and P14ARF alterations in cirrhotic livers.We suggest that the changes impair the regulatory MDM2-P14ARF system, thus possibly favouring regenerative proliferation and transformation.

View Article: PubMed Central - PubMed

Affiliation: Department of Cytopathology, Georg-August-University, Robert-Koch-Strasse 40, D-37075 Goettingen, Germany. tschlott@med.uni-goettingen.de

Show MeSH
Related in: MedlinePlus