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Inhibition of human chondrosarcoma cell growth via apoptosis by peroxisome proliferator-activated receptor-gamma.

Nishida K, Furumatsu T, Takada I, Kawai A, Yoshida A, Kunisada T, Inoue H - Br. J. Cancer (2002)

Bottom Line: Furthermore, the effects of peroxisome proliferator-activated receptor-gamma ligands on cell proliferation and survival were investigated in OUMS-27 cells.The mechanism of cytotoxic effects of 15d-PGJ(2) was via apoptosis as shown by DNA fragmentation using TUNEL stain and DNA ladder formation, and by ultrastructural analysis using transmission electron microscopy.Flow-cytometric analysis using annexin-V-fluorescein and propidium iodide detected the early change of apoptosis, as well as necrosis of OUMS-27 cells at 4 h after co-incubation with 15d-PGJ(2).

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama City, Okayama 700-8558, Japan. knishida@md.okayama-u.ac.jp

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Cell morphology after incubation with or without 15d-PGJ2. The cells were treated by 10 μg ml−2 of 15d-PGJ2 for 8 h, and the cell pellet was embedded in hydrophilic resin. Semi-thin sections stained by toluidine blue show cell shrinkage and nuclear condensation (arrow heads) (B). TEM analysis of ultra-thin section after metal stain revealed typical apoptotic feature. Cells were small, and contained pyknotic nuclei, electron-dense granules and many vacuoles (D). (A and C) Control specimens without stimulation by 15d-PGJ2. Bar=5 μm.
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fig5: Cell morphology after incubation with or without 15d-PGJ2. The cells were treated by 10 μg ml−2 of 15d-PGJ2 for 8 h, and the cell pellet was embedded in hydrophilic resin. Semi-thin sections stained by toluidine blue show cell shrinkage and nuclear condensation (arrow heads) (B). TEM analysis of ultra-thin section after metal stain revealed typical apoptotic feature. Cells were small, and contained pyknotic nuclei, electron-dense granules and many vacuoles (D). (A and C) Control specimens without stimulation by 15d-PGJ2. Bar=5 μm.

Mentions: Induction of apoptosis by 15d-PGJ2 in OUMS-27. (A) OUMS-27 cells were incubated with or without 15d-PGJ2 (10 μg ml−1) for 24 h. Cells were doubly stained by Hoechst 33258 and simultaneously analysed by TUNEL stain, and observed by fluorescent microscopy. The cells with morphologically condensed nucleus were TUNEL positive, indicating the existence of fragmented DNA. (B) DNA was isolated TUNEL positive, indicating the existence of fragmented DNA. (B) DNA was isolated from the cells after co-incubation with 15d-PGJ2 (10 μg ml−1) for 24 h. A clear DNA-ladder formation was obtained after electrophoresis on a 2% agarose gel. M: marker.


Inhibition of human chondrosarcoma cell growth via apoptosis by peroxisome proliferator-activated receptor-gamma.

Nishida K, Furumatsu T, Takada I, Kawai A, Yoshida A, Kunisada T, Inoue H - Br. J. Cancer (2002)

Cell morphology after incubation with or without 15d-PGJ2. The cells were treated by 10 μg ml−2 of 15d-PGJ2 for 8 h, and the cell pellet was embedded in hydrophilic resin. Semi-thin sections stained by toluidine blue show cell shrinkage and nuclear condensation (arrow heads) (B). TEM analysis of ultra-thin section after metal stain revealed typical apoptotic feature. Cells were small, and contained pyknotic nuclei, electron-dense granules and many vacuoles (D). (A and C) Control specimens without stimulation by 15d-PGJ2. Bar=5 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375347&req=5

fig5: Cell morphology after incubation with or without 15d-PGJ2. The cells were treated by 10 μg ml−2 of 15d-PGJ2 for 8 h, and the cell pellet was embedded in hydrophilic resin. Semi-thin sections stained by toluidine blue show cell shrinkage and nuclear condensation (arrow heads) (B). TEM analysis of ultra-thin section after metal stain revealed typical apoptotic feature. Cells were small, and contained pyknotic nuclei, electron-dense granules and many vacuoles (D). (A and C) Control specimens without stimulation by 15d-PGJ2. Bar=5 μm.
Mentions: Induction of apoptosis by 15d-PGJ2 in OUMS-27. (A) OUMS-27 cells were incubated with or without 15d-PGJ2 (10 μg ml−1) for 24 h. Cells were doubly stained by Hoechst 33258 and simultaneously analysed by TUNEL stain, and observed by fluorescent microscopy. The cells with morphologically condensed nucleus were TUNEL positive, indicating the existence of fragmented DNA. (B) DNA was isolated TUNEL positive, indicating the existence of fragmented DNA. (B) DNA was isolated from the cells after co-incubation with 15d-PGJ2 (10 μg ml−1) for 24 h. A clear DNA-ladder formation was obtained after electrophoresis on a 2% agarose gel. M: marker.

Bottom Line: Furthermore, the effects of peroxisome proliferator-activated receptor-gamma ligands on cell proliferation and survival were investigated in OUMS-27 cells.The mechanism of cytotoxic effects of 15d-PGJ(2) was via apoptosis as shown by DNA fragmentation using TUNEL stain and DNA ladder formation, and by ultrastructural analysis using transmission electron microscopy.Flow-cytometric analysis using annexin-V-fluorescein and propidium iodide detected the early change of apoptosis, as well as necrosis of OUMS-27 cells at 4 h after co-incubation with 15d-PGJ(2).

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama City, Okayama 700-8558, Japan. knishida@md.okayama-u.ac.jp

Show MeSH
Related in: MedlinePlus