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Aminolaevulinic acid-induced photodynamic therapy: cellular responses to glucose starvation.

Wyld L, Tomlinson M, Reed MW, Brown NJ - Br. J. Cancer (2002)

Bottom Line: IX measured by spectrofluorimetry.Percentage cell survival was determined by clonogenic assay.Cells cultured in low glucose generated higher levels of protoporphyrin IX compared to standard glucose medium (0 mM glucose: 0.88 x 10(-5) ng cell(-1), 1 mM: 0.86 x 10(-5) ng cell(-1), 25 mM: 0.60 5x 10(-5) ng cell(-1), P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Section of Surgical and Anaesthetic Sciences, Division of Clinical Sciences, University of Sheffield, Floor K, Royal Hallamshire Hospital, Sheffield S10 2JF, UK.

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Graph showing cell growth curves for MCF-7 cells cultured in different glucose medium. Mean number of cells per well at different time intervals after plating. Data shown represent means plus standard error of mean. n=9. *Denotes statistical significance compared to 25 mM glucose cultures, P <0.05, Mann–Whitney U-test.
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fig1: Graph showing cell growth curves for MCF-7 cells cultured in different glucose medium. Mean number of cells per well at different time intervals after plating. Data shown represent means plus standard error of mean. n=9. *Denotes statistical significance compared to 25 mM glucose cultures, P <0.05, Mann–Whitney U-test.

Mentions: Cells cultured in 25 mM glucose commenced active proliferation within 48 h of plating which reached a maximum at day 6, followed by a sudden decrease in cell numbers. This was accompanied by a visible change in the pH of the culture medium, suggesting a build up of lactic acid. For cells cultured under reduced glucose concentrations, 0 and 1 mM, the increase in cell numbers was delayed and peaked at day 5 at a significantly lower level than in the 25 mM glucose medium (day 5 mean cell counts: 0 mM glucose: 3.83×105 cells ml−1, 1 mM glucose: 9.62×105 cells ml−1 and 25 mM glucose: 29.26×105 cells ml−1, P<0.05, Figure 1Figure 1


Aminolaevulinic acid-induced photodynamic therapy: cellular responses to glucose starvation.

Wyld L, Tomlinson M, Reed MW, Brown NJ - Br. J. Cancer (2002)

Graph showing cell growth curves for MCF-7 cells cultured in different glucose medium. Mean number of cells per well at different time intervals after plating. Data shown represent means plus standard error of mean. n=9. *Denotes statistical significance compared to 25 mM glucose cultures, P <0.05, Mann–Whitney U-test.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2375344&req=5

fig1: Graph showing cell growth curves for MCF-7 cells cultured in different glucose medium. Mean number of cells per well at different time intervals after plating. Data shown represent means plus standard error of mean. n=9. *Denotes statistical significance compared to 25 mM glucose cultures, P <0.05, Mann–Whitney U-test.
Mentions: Cells cultured in 25 mM glucose commenced active proliferation within 48 h of plating which reached a maximum at day 6, followed by a sudden decrease in cell numbers. This was accompanied by a visible change in the pH of the culture medium, suggesting a build up of lactic acid. For cells cultured under reduced glucose concentrations, 0 and 1 mM, the increase in cell numbers was delayed and peaked at day 5 at a significantly lower level than in the 25 mM glucose medium (day 5 mean cell counts: 0 mM glucose: 3.83×105 cells ml−1, 1 mM glucose: 9.62×105 cells ml−1 and 25 mM glucose: 29.26×105 cells ml−1, P<0.05, Figure 1Figure 1

Bottom Line: IX measured by spectrofluorimetry.Percentage cell survival was determined by clonogenic assay.Cells cultured in low glucose generated higher levels of protoporphyrin IX compared to standard glucose medium (0 mM glucose: 0.88 x 10(-5) ng cell(-1), 1 mM: 0.86 x 10(-5) ng cell(-1), 25 mM: 0.60 5x 10(-5) ng cell(-1), P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Section of Surgical and Anaesthetic Sciences, Division of Clinical Sciences, University of Sheffield, Floor K, Royal Hallamshire Hospital, Sheffield S10 2JF, UK.

Show MeSH
Related in: MedlinePlus