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Molecular changes in the expression of human colonic nutrient transporters during the transition from normality to malignancy.

Lambert DW, Wood IS, Ellis A, Shirazi-Beechey SP - Br. J. Cancer (2002)

Bottom Line: Butyrate has profound effects on differentiation, proliferation and apoptosis of colonic epithelial cells by regulating expression of various genes associated with these processes.This was reflected in a corresponding reduction in MCT1 mRNA expression, as measured by Northern analysis.Carcinoma samples displaying reduced levels of MCT1 were found to express the high affinity glucose transporter, GLUT1, suggesting that there is a switch from butyrate to glucose as an energy source in colonic epithelia during transition to malignancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, UK.

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Related in: MedlinePlus

Detection of MCT1 mRNA in healthy human colonic biopsy sections by in situ hybridisation. In situ hybridisation was carried out on healthy human colon biopsy sections using DIG labelled cRNA probes as described in the Materials and Methods section. Sections were hybridised with a specific antisense probe for MCT1 and the corresponding sense probe to act as a control for non-specific binding. (A) MCT1 antisense probe (×200); (B) MCT1 antisense probe (×400); (C) sense probe (×200); (D) MCT1 antisense probe on RNase pre-treated sections (×200). Specific staining is seen as a dark purple colour, contrasting with the blue/green counterstained nuclei. Bars represent 50 μm.
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fig2: Detection of MCT1 mRNA in healthy human colonic biopsy sections by in situ hybridisation. In situ hybridisation was carried out on healthy human colon biopsy sections using DIG labelled cRNA probes as described in the Materials and Methods section. Sections were hybridised with a specific antisense probe for MCT1 and the corresponding sense probe to act as a control for non-specific binding. (A) MCT1 antisense probe (×200); (B) MCT1 antisense probe (×400); (C) sense probe (×200); (D) MCT1 antisense probe on RNase pre-treated sections (×200). Specific staining is seen as a dark purple colour, contrasting with the blue/green counterstained nuclei. Bars represent 50 μm.

Mentions: We next sought to investigate the profile of expression of the MCT1 transcript along the crypt-surface axis. In situ hybridisation histochemistry, using a DIG-labelled antisense probe specific for MCT1, indicated that the MCT1 transcript is expressed along the length of the crypt-surface axis (Figure 2AFigure 2


Molecular changes in the expression of human colonic nutrient transporters during the transition from normality to malignancy.

Lambert DW, Wood IS, Ellis A, Shirazi-Beechey SP - Br. J. Cancer (2002)

Detection of MCT1 mRNA in healthy human colonic biopsy sections by in situ hybridisation. In situ hybridisation was carried out on healthy human colon biopsy sections using DIG labelled cRNA probes as described in the Materials and Methods section. Sections were hybridised with a specific antisense probe for MCT1 and the corresponding sense probe to act as a control for non-specific binding. (A) MCT1 antisense probe (×200); (B) MCT1 antisense probe (×400); (C) sense probe (×200); (D) MCT1 antisense probe on RNase pre-treated sections (×200). Specific staining is seen as a dark purple colour, contrasting with the blue/green counterstained nuclei. Bars represent 50 μm.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2375337&req=5

fig2: Detection of MCT1 mRNA in healthy human colonic biopsy sections by in situ hybridisation. In situ hybridisation was carried out on healthy human colon biopsy sections using DIG labelled cRNA probes as described in the Materials and Methods section. Sections were hybridised with a specific antisense probe for MCT1 and the corresponding sense probe to act as a control for non-specific binding. (A) MCT1 antisense probe (×200); (B) MCT1 antisense probe (×400); (C) sense probe (×200); (D) MCT1 antisense probe on RNase pre-treated sections (×200). Specific staining is seen as a dark purple colour, contrasting with the blue/green counterstained nuclei. Bars represent 50 μm.
Mentions: We next sought to investigate the profile of expression of the MCT1 transcript along the crypt-surface axis. In situ hybridisation histochemistry, using a DIG-labelled antisense probe specific for MCT1, indicated that the MCT1 transcript is expressed along the length of the crypt-surface axis (Figure 2AFigure 2

Bottom Line: Butyrate has profound effects on differentiation, proliferation and apoptosis of colonic epithelial cells by regulating expression of various genes associated with these processes.This was reflected in a corresponding reduction in MCT1 mRNA expression, as measured by Northern analysis.Carcinoma samples displaying reduced levels of MCT1 were found to express the high affinity glucose transporter, GLUT1, suggesting that there is a switch from butyrate to glucose as an energy source in colonic epithelia during transition to malignancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, UK.

Show MeSH
Related in: MedlinePlus