Molecular changes in the expression of human colonic nutrient transporters during the transition from normality to malignancy.
Bottom Line: Butyrate has profound effects on differentiation, proliferation and apoptosis of colonic epithelial cells by regulating expression of various genes associated with these processes.This was reflected in a corresponding reduction in MCT1 mRNA expression, as measured by Northern analysis.Carcinoma samples displaying reduced levels of MCT1 were found to express the high affinity glucose transporter, GLUT1, suggesting that there is a switch from butyrate to glucose as an energy source in colonic epithelia during transition to malignancy.
Affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, UK.Show MeSH
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Mentions: We next sought to investigate the profile of expression of the MCT1 transcript along the crypt-surface axis. In situ hybridisation histochemistry, using a DIG-labelled antisense probe specific for MCT1, indicated that the MCT1 transcript is expressed along the length of the crypt-surface axis (Figure 2AFigure 2
Affiliation: Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, UK.