Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression.
Bottom Line: Interestingly, the expression of cyclin B1 was inhibited to about 60% in U87.Mxi1.14 cells.This inhibition occurs at the transcriptional level and depends, at least in part, on the E-box present on the cyclin B1 promoter.Consistent with this, the endogenous Mxi1 binds this E-box in vitro.
Affiliation: Istituto Regina Elena, Centro Ricerca Sperimentale, Laboratorio di Oncogenesi Molecolare, Via delle messi D'Oro 156, 00158 Rome, Italy.Show MeSH
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Mentions: We previously demonstrated that the Max protein recognizes the E-box present on the cyclin B1 promoter (Farina et al, 1996). By EMSAs we verified the ability of Mxi1 to bind the cyclin B1 E-box. Electromobility shift assays, performed with U87 nuclear extracts, reveal one protein complex binding to the radiolabelled probe, spanning nt −133 to −110 in the cyclin B1 promoter (B1 E-box) (Figure 8Figure 8
Affiliation: Istituto Regina Elena, Centro Ricerca Sperimentale, Laboratorio di Oncogenesi Molecolare, Via delle messi D'Oro 156, 00158 Rome, Italy.