S100A4 (p9Ka) protein in colon carcinoma and liver metastases: association with carcinoma cells and T-lymphocytes.
Bottom Line: In colon cancer, primary tumours as well as metastases to the liver can be studied.Here we show, using quantitative PCR applied to RNA from 24 normal colon, four liver tissues, 24 colon carcinoma specimens, and 24 livers containing colonic carcinoma metastases, that the level of S100A4 mRNA was significantly higher in the carcinomas compared to normal specimens (Mann-Whitney U-test, P=0.05), and in liver metastases compared to carcinoma specimens (P=0.039).The latter comparison included seven liver metastases and their matched primary carcinomas (P<0.001) from the same patient.
Affiliation: Cancer Tissue Bank Research Centre, University of Liverpool, P.O. Box 147, Liverpool, L69 7ZB, UK.Show MeSH
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Mentions: In order to find out whether a particular subtype of lymphocytes contained the S100A4, serial sections of some tumour specimens were immunocytochemically stained with antibodies to S100A4, and also with antibodies to CD3 and CD79a antigens for T and B lymphocytes, respectively. S100A4 appeared to be associated with T lymphocytes (not shown), but the close association of T and B lymphocytes in the tumours prevented a firm conclusion to be drawn. Thus, serial sections of normal specimens of a human tissue with a characteristic distribution of T and B lymphocytes, namely human appendix, were similarly stained. S100A4 clearly co-localized to the region of the appendix containing predominantly immunocytochemically-determined T cells but not to the region containing predominantly immunocytochemically-determined B cells (Figure 5Figure 5
Affiliation: Cancer Tissue Bank Research Centre, University of Liverpool, P.O. Box 147, Liverpool, L69 7ZB, UK.