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Local hypoxia is produced at sites of intratumour injection.

Olive PL, Luo CM, Banáth JP - Br. J. Cancer (2002)

Bottom Line: Hoechst-labelled cells sorted from these tumours were more sensitive to killing by hypoxic cell cytotoxins (tirapazamine, RSU-1069) and less sensitive to damage by ionizing radiation.Hoechst-labelled cells also bound the hypoxia marker pimonidazole when given by i.p. injection.This effect could have significant implications for intratumour injection of drugs, cytokines or vectors that are affected by the oxygenation status of the tumour cells as well as potential effects on biodistribution via local microvasculature.

View Article: PubMed Central - PubMed

Affiliation: Medical Biophysics Department, British Columbia Cancer Research Centre, 601 W 10th Avenue, Vancouver, BC, V5Z 1L3 Canada. polive@bccancer.bc.ca

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SCCVII tumours (400–500 mg) were injected with 20 ng Hoechst 33342 in 0.1 ml along three tracks within the tumour. Tumours were removed at the times indicated, and a single cell suspension was prepared for analysis using a Becton-Dickinson FACS 440 cell sorter. The solid circles indicate the distribution of 1600 ng Hoechst 33342 given by a single i.v. injection.
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fig1: SCCVII tumours (400–500 mg) were injected with 20 ng Hoechst 33342 in 0.1 ml along three tracks within the tumour. Tumours were removed at the times indicated, and a single cell suspension was prepared for analysis using a Becton-Dickinson FACS 440 cell sorter. The solid circles indicate the distribution of 1600 ng Hoechst 33342 given by a single i.v. injection.

Mentions: Figure 1Figure 1


Local hypoxia is produced at sites of intratumour injection.

Olive PL, Luo CM, Banáth JP - Br. J. Cancer (2002)

SCCVII tumours (400–500 mg) were injected with 20 ng Hoechst 33342 in 0.1 ml along three tracks within the tumour. Tumours were removed at the times indicated, and a single cell suspension was prepared for analysis using a Becton-Dickinson FACS 440 cell sorter. The solid circles indicate the distribution of 1600 ng Hoechst 33342 given by a single i.v. injection.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375199&req=5

fig1: SCCVII tumours (400–500 mg) were injected with 20 ng Hoechst 33342 in 0.1 ml along three tracks within the tumour. Tumours were removed at the times indicated, and a single cell suspension was prepared for analysis using a Becton-Dickinson FACS 440 cell sorter. The solid circles indicate the distribution of 1600 ng Hoechst 33342 given by a single i.v. injection.
Mentions: Figure 1Figure 1

Bottom Line: Hoechst-labelled cells sorted from these tumours were more sensitive to killing by hypoxic cell cytotoxins (tirapazamine, RSU-1069) and less sensitive to damage by ionizing radiation.Hoechst-labelled cells also bound the hypoxia marker pimonidazole when given by i.p. injection.This effect could have significant implications for intratumour injection of drugs, cytokines or vectors that are affected by the oxygenation status of the tumour cells as well as potential effects on biodistribution via local microvasculature.

View Article: PubMed Central - PubMed

Affiliation: Medical Biophysics Department, British Columbia Cancer Research Centre, 601 W 10th Avenue, Vancouver, BC, V5Z 1L3 Canada. polive@bccancer.bc.ca

Show MeSH
Related in: MedlinePlus