Limits...
E7 proteins from oncogenic human papillomavirus types transactivate p73: role in cervical intraepithelial neoplasia.

Brooks LA, Sullivan A, O'Nions J, Bell A, Dunne B, Tidy JA, Evans DJ, Osin P, Vousden KH, Gusterson B, Farrell PJ, Storey A, Gasco M, Sakai T, Crook T - Br. J. Cancer (2002)

Bottom Line: Expression of p73 in normal cervical epithelium is confined to the basal and supra-basal layers.In contrast, expression in neoplastic lesions is detected throughout the epithelium and increases with grade of neoplasia, being maximal in squamous cell cancers (SCC).The frequent over-expression of p73Delta2, which has recognized transdominant properties, in malignant and pre-malignant lesions suggests a role in the oncogenic process in cervical epithelium.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, Norfolk Place, London W2 1PG, UK.

Show MeSH

Related in: MedlinePlus

Expression of p14ARFand p73 in cervical epithelium. RT–PCR was performed as described in Materials and Methods. (A) p73 is over-expressed in HPV 16 positive cervical SCC. A single variant of p73 (p73α) is predominantly expressed in both normal (N) and malignant (T) cervical epithelium. The presence of equivalent amounts of cDNA in each sample was verified by amplification of β-actin as shown. (B) Cervical SCC express both full-length and Δ2 forms of p73. RT–PCR for p73Δ2 was performed as described in Materials and Methods. Amplification products were resolved on 2% agarose gels, then hybridized with oligonucleotide probes derived from exon 2 (upper blot) or exon 3 (lower blot). Hybridization with the probe from exon 2 detects only the full-length transcript, whereas the probe derived from exon 3 detects both the full-length and Δ2 forms of p73. In all cases N=normal tissue, T=tumour. Lanes N6 to T9 are four matched cases of normal and tumour. Lanes 10–13 are four examples of cervical SCC, shown without matched normal tissue. The presence of equivalent amounts of cDNA in each reaction was confirmed by amplification of β-actin as shown in the lower panel.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2375198&req=5

fig3: Expression of p14ARFand p73 in cervical epithelium. RT–PCR was performed as described in Materials and Methods. (A) p73 is over-expressed in HPV 16 positive cervical SCC. A single variant of p73 (p73α) is predominantly expressed in both normal (N) and malignant (T) cervical epithelium. The presence of equivalent amounts of cDNA in each sample was verified by amplification of β-actin as shown. (B) Cervical SCC express both full-length and Δ2 forms of p73. RT–PCR for p73Δ2 was performed as described in Materials and Methods. Amplification products were resolved on 2% agarose gels, then hybridized with oligonucleotide probes derived from exon 2 (upper blot) or exon 3 (lower blot). Hybridization with the probe from exon 2 detects only the full-length transcript, whereas the probe derived from exon 3 detects both the full-length and Δ2 forms of p73. In all cases N=normal tissue, T=tumour. Lanes N6 to T9 are four matched cases of normal and tumour. Lanes 10–13 are four examples of cervical SCC, shown without matched normal tissue. The presence of equivalent amounts of cDNA in each reaction was confirmed by amplification of β-actin as shown in the lower panel.

Mentions: To address the relevance of the results obtained from transient assays and cell lines, we performed RT–PCR and immunocytochemical analysis of p73 expression in primary cervical tissues, these comprising a series of HPV 16 positive CIN I, CIN III and cervical SCC (Figures 3Figure 3


E7 proteins from oncogenic human papillomavirus types transactivate p73: role in cervical intraepithelial neoplasia.

Brooks LA, Sullivan A, O'Nions J, Bell A, Dunne B, Tidy JA, Evans DJ, Osin P, Vousden KH, Gusterson B, Farrell PJ, Storey A, Gasco M, Sakai T, Crook T - Br. J. Cancer (2002)

Expression of p14ARFand p73 in cervical epithelium. RT–PCR was performed as described in Materials and Methods. (A) p73 is over-expressed in HPV 16 positive cervical SCC. A single variant of p73 (p73α) is predominantly expressed in both normal (N) and malignant (T) cervical epithelium. The presence of equivalent amounts of cDNA in each sample was verified by amplification of β-actin as shown. (B) Cervical SCC express both full-length and Δ2 forms of p73. RT–PCR for p73Δ2 was performed as described in Materials and Methods. Amplification products were resolved on 2% agarose gels, then hybridized with oligonucleotide probes derived from exon 2 (upper blot) or exon 3 (lower blot). Hybridization with the probe from exon 2 detects only the full-length transcript, whereas the probe derived from exon 3 detects both the full-length and Δ2 forms of p73. In all cases N=normal tissue, T=tumour. Lanes N6 to T9 are four matched cases of normal and tumour. Lanes 10–13 are four examples of cervical SCC, shown without matched normal tissue. The presence of equivalent amounts of cDNA in each reaction was confirmed by amplification of β-actin as shown in the lower panel.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375198&req=5

fig3: Expression of p14ARFand p73 in cervical epithelium. RT–PCR was performed as described in Materials and Methods. (A) p73 is over-expressed in HPV 16 positive cervical SCC. A single variant of p73 (p73α) is predominantly expressed in both normal (N) and malignant (T) cervical epithelium. The presence of equivalent amounts of cDNA in each sample was verified by amplification of β-actin as shown. (B) Cervical SCC express both full-length and Δ2 forms of p73. RT–PCR for p73Δ2 was performed as described in Materials and Methods. Amplification products were resolved on 2% agarose gels, then hybridized with oligonucleotide probes derived from exon 2 (upper blot) or exon 3 (lower blot). Hybridization with the probe from exon 2 detects only the full-length transcript, whereas the probe derived from exon 3 detects both the full-length and Δ2 forms of p73. In all cases N=normal tissue, T=tumour. Lanes N6 to T9 are four matched cases of normal and tumour. Lanes 10–13 are four examples of cervical SCC, shown without matched normal tissue. The presence of equivalent amounts of cDNA in each reaction was confirmed by amplification of β-actin as shown in the lower panel.
Mentions: To address the relevance of the results obtained from transient assays and cell lines, we performed RT–PCR and immunocytochemical analysis of p73 expression in primary cervical tissues, these comprising a series of HPV 16 positive CIN I, CIN III and cervical SCC (Figures 3Figure 3

Bottom Line: Expression of p73 in normal cervical epithelium is confined to the basal and supra-basal layers.In contrast, expression in neoplastic lesions is detected throughout the epithelium and increases with grade of neoplasia, being maximal in squamous cell cancers (SCC).The frequent over-expression of p73Delta2, which has recognized transdominant properties, in malignant and pre-malignant lesions suggests a role in the oncogenic process in cervical epithelium.

View Article: PubMed Central - PubMed

Affiliation: Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, Norfolk Place, London W2 1PG, UK.

Show MeSH
Related in: MedlinePlus