Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.
Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.
Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.Show MeSH
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Mentions: We then assessed whether the loss of ER-α expression in the cell lines was associated with DNA methylation in the CpG island of the ER gene using MS–PCR with primers designed to identify the NotI site of the ER-α promoter. The results of these amplifications are shown in Figure 2Figure 2
Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.