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Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.

O'Doherty AM, Church SW, Russell SE, Nelson J, Hickey I - Br. J. Cancer (2002)

Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.

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Related in: MedlinePlus

(A) MS–PCR of Na bisulphite-treated DNA from cell lines. Lanes 1, 2: PEO 1; lanes 2, 3: PEO 14; lanes 5, 6: A2780; lanes 7, 8: ONT 14; lanes 9, 10: OAW 42; lanes 11, 12: PEO 4; lanes 13, 14: MCF-7. Unmethylated reactions: lanes 1, 3, 5, 7, 9, 11 and 13. Methylated reactions: lanes 2, 4, 6, 8, 10, 12 and 14. M, 100 bp marker. (B) MS–PCR of Na bisulphite treated DNA from cell line PEA 1. Lane 1, unmethylated reaction; lane 2, methylated reaction; M, 100 bp ladder.
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fig2: (A) MS–PCR of Na bisulphite-treated DNA from cell lines. Lanes 1, 2: PEO 1; lanes 2, 3: PEO 14; lanes 5, 6: A2780; lanes 7, 8: ONT 14; lanes 9, 10: OAW 42; lanes 11, 12: PEO 4; lanes 13, 14: MCF-7. Unmethylated reactions: lanes 1, 3, 5, 7, 9, 11 and 13. Methylated reactions: lanes 2, 4, 6, 8, 10, 12 and 14. M, 100 bp marker. (B) MS–PCR of Na bisulphite treated DNA from cell line PEA 1. Lane 1, unmethylated reaction; lane 2, methylated reaction; M, 100 bp ladder.

Mentions: We then assessed whether the loss of ER-α expression in the cell lines was associated with DNA methylation in the CpG island of the ER gene using MS–PCR with primers designed to identify the NotI site of the ER-α promoter. The results of these amplifications are shown in Figure 2Figure 2


Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.

O'Doherty AM, Church SW, Russell SE, Nelson J, Hickey I - Br. J. Cancer (2002)

(A) MS–PCR of Na bisulphite-treated DNA from cell lines. Lanes 1, 2: PEO 1; lanes 2, 3: PEO 14; lanes 5, 6: A2780; lanes 7, 8: ONT 14; lanes 9, 10: OAW 42; lanes 11, 12: PEO 4; lanes 13, 14: MCF-7. Unmethylated reactions: lanes 1, 3, 5, 7, 9, 11 and 13. Methylated reactions: lanes 2, 4, 6, 8, 10, 12 and 14. M, 100 bp marker. (B) MS–PCR of Na bisulphite treated DNA from cell line PEA 1. Lane 1, unmethylated reaction; lane 2, methylated reaction; M, 100 bp ladder.
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Related In: Results  -  Collection

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fig2: (A) MS–PCR of Na bisulphite-treated DNA from cell lines. Lanes 1, 2: PEO 1; lanes 2, 3: PEO 14; lanes 5, 6: A2780; lanes 7, 8: ONT 14; lanes 9, 10: OAW 42; lanes 11, 12: PEO 4; lanes 13, 14: MCF-7. Unmethylated reactions: lanes 1, 3, 5, 7, 9, 11 and 13. Methylated reactions: lanes 2, 4, 6, 8, 10, 12 and 14. M, 100 bp marker. (B) MS–PCR of Na bisulphite treated DNA from cell line PEA 1. Lane 1, unmethylated reaction; lane 2, methylated reaction; M, 100 bp ladder.
Mentions: We then assessed whether the loss of ER-α expression in the cell lines was associated with DNA methylation in the CpG island of the ER gene using MS–PCR with primers designed to identify the NotI site of the ER-α promoter. The results of these amplifications are shown in Figure 2Figure 2

Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.

Show MeSH
Related in: MedlinePlus