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Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.

O'Doherty AM, Church SW, Russell SE, Nelson J, Hickey I - Br. J. Cancer (2002)

Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.

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(A) Western blot of protein extracts from ovarian cell lines probed with antibody against ER-α Lanes: 1, PEO1; 2, PEO14; 3, A2780; 4, OTN14; 5, OAW42; 6, PEO4; 7, MCF-7. (B) The same blot stripped and reprobed with antibody against tubulin.
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fig1: (A) Western blot of protein extracts from ovarian cell lines probed with antibody against ER-α Lanes: 1, PEO1; 2, PEO14; 3, A2780; 4, OTN14; 5, OAW42; 6, PEO4; 7, MCF-7. (B) The same blot stripped and reprobed with antibody against tubulin.

Mentions: We analyzed the ER-α status of six epithelial ovarian cell lines by Western blotting. The well-characterized breast cancer cell line MCF-7 was used as a positive control. The data presented in Figure 1AFigure 1


Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.

O'Doherty AM, Church SW, Russell SE, Nelson J, Hickey I - Br. J. Cancer (2002)

(A) Western blot of protein extracts from ovarian cell lines probed with antibody against ER-α Lanes: 1, PEO1; 2, PEO14; 3, A2780; 4, OTN14; 5, OAW42; 6, PEO4; 7, MCF-7. (B) The same blot stripped and reprobed with antibody against tubulin.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2375192&req=5

fig1: (A) Western blot of protein extracts from ovarian cell lines probed with antibody against ER-α Lanes: 1, PEO1; 2, PEO14; 3, A2780; 4, OTN14; 5, OAW42; 6, PEO4; 7, MCF-7. (B) The same blot stripped and reprobed with antibody against tubulin.
Mentions: We analyzed the ER-α status of six epithelial ovarian cell lines by Western blotting. The well-characterized breast cancer cell line MCF-7 was used as a positive control. The data presented in Figure 1AFigure 1

Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.

View Article: PubMed Central - PubMed

Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.

Show MeSH
Related in: MedlinePlus