Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.
Bottom Line: Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation.In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island.These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.
Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.Show MeSH
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Mentions: We analyzed the ER-α status of six epithelial ovarian cell lines by Western blotting. The well-characterized breast cancer cell line MCF-7 was used as a positive control. The data presented in Figure 1AFigure 1
Affiliation: School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.