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Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Bottom Line: Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Expression of oestrogen receptor beta was exclusively nuclear and occurred in multiple cell types.There was no quantitative relationship between staining for the two ERs although in tumours in which both receptors were present immunoexpression of oestrogen receptor alpha was invariably more intense.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

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Quantification of immunoexpression for ER isotypes. Box and whisker plot summarising the relationship between score for ERα (x-axis) and ERβ (y-axis) for each sample. Solid horizontal line shows the median for the data, the top of the box the 25th percentile, the bottom the 75th percentile and the additional lines the range of the data. Note that there were no samples with an ERα score of 1 to 5.
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fig5: Quantification of immunoexpression for ER isotypes. Box and whisker plot summarising the relationship between score for ERα (x-axis) and ERβ (y-axis) for each sample. Solid horizontal line shows the median for the data, the top of the box the 25th percentile, the bottom the 75th percentile and the additional lines the range of the data. Note that there were no samples with an ERα score of 1 to 5.

Mentions: Most of the tumours (48 out of 51) displayed staining for ERβ in malignant epithelium with a range of scoring between 2 and 7 (median score 4.5). ERα staining was found in 41 out of 50 tumours with a range of scoring between 6 and 8 (median score 7.5). Quantitatively it was possible to identify ERα-positive, ERβ-positive tumours (38 out of 51, Figures 3A and 4A) as well as ERα-positive, ERβ-negative tumours (3 out of 51, Figure 3B compared with Figure 4B; 2 out of 51). ERα-negative, ERβ-positive tumours were detected (10 out of 51) but we observed no double negatives. There was no quantitative relationship between immunohistochemical scores for ERα and ERβ (Figure 5Figure 5


Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Quantification of immunoexpression for ER isotypes. Box and whisker plot summarising the relationship between score for ERα (x-axis) and ERβ (y-axis) for each sample. Solid horizontal line shows the median for the data, the top of the box the 25th percentile, the bottom the 75th percentile and the additional lines the range of the data. Note that there were no samples with an ERα score of 1 to 5.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375186&req=5

fig5: Quantification of immunoexpression for ER isotypes. Box and whisker plot summarising the relationship between score for ERα (x-axis) and ERβ (y-axis) for each sample. Solid horizontal line shows the median for the data, the top of the box the 25th percentile, the bottom the 75th percentile and the additional lines the range of the data. Note that there were no samples with an ERα score of 1 to 5.
Mentions: Most of the tumours (48 out of 51) displayed staining for ERβ in malignant epithelium with a range of scoring between 2 and 7 (median score 4.5). ERα staining was found in 41 out of 50 tumours with a range of scoring between 6 and 8 (median score 7.5). Quantitatively it was possible to identify ERα-positive, ERβ-positive tumours (38 out of 51, Figures 3A and 4A) as well as ERα-positive, ERβ-negative tumours (3 out of 51, Figure 3B compared with Figure 4B; 2 out of 51). ERα-negative, ERβ-positive tumours were detected (10 out of 51) but we observed no double negatives. There was no quantitative relationship between immunohistochemical scores for ERα and ERβ (Figure 5Figure 5

Bottom Line: Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Expression of oestrogen receptor beta was exclusively nuclear and occurred in multiple cell types.There was no quantitative relationship between staining for the two ERs although in tumours in which both receptors were present immunoexpression of oestrogen receptor alpha was invariably more intense.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

Show MeSH
Related in: MedlinePlus