Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.
Bottom Line: The anti-oestrogen receptor beta antibody cross reacted specifically with both long (approximately 59 Kd) and short (approximately 53 Kd) forms of recombinant oestrogen receptor beta.Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Fifty-one breast cancer biopsies were examined using immunohistochemistry; 41 (80%) were immunopositive for oestrogen receptor alpha, 48 (94%) were immunopositive for oestrogen receptor beta and 38 (74.5%) co-expressed both receptors.
Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. firstname.lastname@example.orgShow MeSH
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Mentions: Most of the tumours (48 out of 51) displayed staining for ERβ in malignant epithelium with a range of scoring between 2 and 7 (median score 4.5). ERα staining was found in 41 out of 50 tumours with a range of scoring between 6 and 8 (median score 7.5). Quantitatively it was possible to identify ERα-positive, ERβ-positive tumours (38 out of 51, Figures 3A and 4A) as well as ERα-positive, ERβ-negative tumours (3 out of 51, Figure 3B compared with Figure 4B; 2 out of 51). ERα-negative, ERβ-positive tumours were detected (10 out of 51) but we observed no double negatives. There was no quantitative relationship between immunohistochemical scores for ERα and ERβ (Figure 5Figure 5
Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. email@example.com