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Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Bottom Line: The anti-oestrogen receptor beta antibody cross reacted specifically with both long (approximately 59 Kd) and short (approximately 53 Kd) forms of recombinant oestrogen receptor beta.Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Fifty-one breast cancer biopsies were examined using immunohistochemistry; 41 (80%) were immunopositive for oestrogen receptor alpha, 48 (94%) were immunopositive for oestrogen receptor beta and 38 (74.5%) co-expressed both receptors.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

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Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
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fig1: Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.

Mentions: All samples tested (n=9) were positive for ERβ following RT–PCR (Figure 1Figure 1


Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375186&req=5

fig1: Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
Mentions: All samples tested (n=9) were positive for ERβ following RT–PCR (Figure 1Figure 1

Bottom Line: The anti-oestrogen receptor beta antibody cross reacted specifically with both long (approximately 59 Kd) and short (approximately 53 Kd) forms of recombinant oestrogen receptor beta.Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Fifty-one breast cancer biopsies were examined using immunohistochemistry; 41 (80%) were immunopositive for oestrogen receptor alpha, 48 (94%) were immunopositive for oestrogen receptor beta and 38 (74.5%) co-expressed both receptors.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

Show MeSH
Related in: MedlinePlus