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Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Bottom Line: Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Expression of oestrogen receptor beta was exclusively nuclear and occurred in multiple cell types.There was no quantitative relationship between staining for the two ERs although in tumours in which both receptors were present immunoexpression of oestrogen receptor alpha was invariably more intense.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

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Related in: MedlinePlus

Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
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fig1: Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.

Mentions: All samples tested (n=9) were positive for ERβ following RT–PCR (Figure 1Figure 1


Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.

Saunders PT, Millar MR, Williams K, Macpherson S, Bayne C, O'Sullivan C, Anderson TJ, Groome NP, Miller WR - Br. J. Cancer (2002)

Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375186&req=5

fig1: Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.
Mentions: All samples tested (n=9) were positive for ERβ following RT–PCR (Figure 1Figure 1

Bottom Line: Western blot analysis of breast tumours contained both forms of oestrogen receptor beta protein although in some samples lower molecular weight species (32--45 Kd) were identified.Expression of oestrogen receptor beta was exclusively nuclear and occurred in multiple cell types.There was no quantitative relationship between staining for the two ERs although in tumours in which both receptors were present immunoexpression of oestrogen receptor alpha was invariably more intense.

View Article: PubMed Central - PubMed

Affiliation: MRC Human Reproductive Sciences Unit, 37 Chalmers Street, Edinburgh, EH3 9ET, UK. p.saunders@ed.ac.uk

Show MeSH
Related in: MedlinePlus