Variants of the long control region and the E6 oncogene in European human papillomavirus type 16 isolates: implications for cervical disease.
Bottom Line: This could partially explain why some lesions progress to cancer while others do not.In addition, we found a specific European E6 variant, L83V, to be enriched in high-grade lesions and cancer rather than a specific European long control region variant.The difference in oncogenicity between European HPV16 genotypes is more probably due to an altered property of the corresponding E6 proteins rather than to an altered activity of the P97 promoter.
Affiliation: Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, D-50935 Cologne, Germany.Show MeSH
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Mentions: The HPV16 LCRs were cloned into the pALuc vector in front of the luciferase gene in order to determine whether the above-mentioned sequence alterations affect the P97 promoter activity. C33A cells were transfected with the isolate constructs and luciferase activities were analyzed 48 h later. All isolates, except US56, exhibited approximately the same activity as the European reference clone with transcription activities ranging from 0.87 to 1.27 (Figure 2Figure 2
Affiliation: Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, D-50935 Cologne, Germany.