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Variants of the long control region and the E6 oncogene in European human papillomavirus type 16 isolates: implications for cervical disease.

Kämmer C, Tommasino M, Syrjänen S, Delius H, Hebling U, Warthorst U, Pfister H, Zehbe I - Br. J. Cancer (2002)

Bottom Line: This could partially explain why some lesions progress to cancer while others do not.In addition, we found a specific European E6 variant, L83V, to be enriched in high-grade lesions and cancer rather than a specific European long control region variant.The difference in oncogenicity between European HPV16 genotypes is more probably due to an altered property of the corresponding E6 proteins rather than to an altered activity of the P97 promoter.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, D-50935 Cologne, Germany.

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Related in: MedlinePlus

Sequence alterations relative to the LCR and E6 open reading frame of reference HPV16. Differences between the isolates and the HPV16 reference sequence (bold) are shown. The type of lesion from which the isolates are derived is indicated. Dashes indicate nucleotides identical to the reference sequence. Capital letters indicate alterations in the E6 open reading frame that result in amino acid changes, while lower case letters indicate silent mutations. The position of amino acid change is indicated numerically. The letter preceding this number refers to the prototype amino acid, and the letter following it refers to its substitution. The polymorphisms not reported previously appear in italics. Swedish cases are denoted ‘US’ and Finnish cases ‘F’ or ‘T’. HPV=human papillomavirus; LCR=long control region; ORF=open reading frame; R=reference sequence (Seedorf et al, 1985); L/HCIN=low-grade/high-grade cervical intraepithelial neoplasia.
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fig1: Sequence alterations relative to the LCR and E6 open reading frame of reference HPV16. Differences between the isolates and the HPV16 reference sequence (bold) are shown. The type of lesion from which the isolates are derived is indicated. Dashes indicate nucleotides identical to the reference sequence. Capital letters indicate alterations in the E6 open reading frame that result in amino acid changes, while lower case letters indicate silent mutations. The position of amino acid change is indicated numerically. The letter preceding this number refers to the prototype amino acid, and the letter following it refers to its substitution. The polymorphisms not reported previously appear in italics. Swedish cases are denoted ‘US’ and Finnish cases ‘F’ or ‘T’. HPV=human papillomavirus; LCR=long control region; ORF=open reading frame; R=reference sequence (Seedorf et al, 1985); L/HCIN=low-grade/high-grade cervical intraepithelial neoplasia.

Mentions: After PCR, the entire HPV16 LCR (nts 7010–124) and the entire HPV16 E6 gene (nts 83–559) were sequenced and compared with the European reference sequence (HPV16R sequence in the HPV database). The sequences of all samples tested in the present investigation are summarized in Figure 1Figure 1


Variants of the long control region and the E6 oncogene in European human papillomavirus type 16 isolates: implications for cervical disease.

Kämmer C, Tommasino M, Syrjänen S, Delius H, Hebling U, Warthorst U, Pfister H, Zehbe I - Br. J. Cancer (2002)

Sequence alterations relative to the LCR and E6 open reading frame of reference HPV16. Differences between the isolates and the HPV16 reference sequence (bold) are shown. The type of lesion from which the isolates are derived is indicated. Dashes indicate nucleotides identical to the reference sequence. Capital letters indicate alterations in the E6 open reading frame that result in amino acid changes, while lower case letters indicate silent mutations. The position of amino acid change is indicated numerically. The letter preceding this number refers to the prototype amino acid, and the letter following it refers to its substitution. The polymorphisms not reported previously appear in italics. Swedish cases are denoted ‘US’ and Finnish cases ‘F’ or ‘T’. HPV=human papillomavirus; LCR=long control region; ORF=open reading frame; R=reference sequence (Seedorf et al, 1985); L/HCIN=low-grade/high-grade cervical intraepithelial neoplasia.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375181&req=5

fig1: Sequence alterations relative to the LCR and E6 open reading frame of reference HPV16. Differences between the isolates and the HPV16 reference sequence (bold) are shown. The type of lesion from which the isolates are derived is indicated. Dashes indicate nucleotides identical to the reference sequence. Capital letters indicate alterations in the E6 open reading frame that result in amino acid changes, while lower case letters indicate silent mutations. The position of amino acid change is indicated numerically. The letter preceding this number refers to the prototype amino acid, and the letter following it refers to its substitution. The polymorphisms not reported previously appear in italics. Swedish cases are denoted ‘US’ and Finnish cases ‘F’ or ‘T’. HPV=human papillomavirus; LCR=long control region; ORF=open reading frame; R=reference sequence (Seedorf et al, 1985); L/HCIN=low-grade/high-grade cervical intraepithelial neoplasia.
Mentions: After PCR, the entire HPV16 LCR (nts 7010–124) and the entire HPV16 E6 gene (nts 83–559) were sequenced and compared with the European reference sequence (HPV16R sequence in the HPV database). The sequences of all samples tested in the present investigation are summarized in Figure 1Figure 1

Bottom Line: This could partially explain why some lesions progress to cancer while others do not.In addition, we found a specific European E6 variant, L83V, to be enriched in high-grade lesions and cancer rather than a specific European long control region variant.The difference in oncogenicity between European HPV16 genotypes is more probably due to an altered property of the corresponding E6 proteins rather than to an altered activity of the P97 promoter.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, University of Cologne, Fürst-Pückler-Strasse 56, D-50935 Cologne, Germany.

Show MeSH
Related in: MedlinePlus