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Recovery of zeta-chain expression and changes in spontaneous IL-10 production after PSA-based vaccines in patients with prostate cancer.

Meidenbauer N, Gooding W, Spitler L, Harris D, Whiteside TL - Br. J. Cancer (2002)

Bottom Line: Prior to therapy, the patients were found to have significantly lower zeta-chain expression in circulating CD3(+) cells and a higher percentage of zeta-chain negative CD3(+) and CD4(+) cells than normal donors.Only one of 18 patients was a clinical responder.The vaccine had stimulatory effects on the patients' immune system, but post-vaccine immune recovery could not be correlated to progression-free survival in this small cohort of patients with prostate cancer.

View Article: PubMed Central - PubMed

Affiliation: University of Pittsburgh Cancer Institute, W1041 Biomedical Science Tower, 211 Lothrop Street, Pittsburgh, Pennsylvania, PA 15213-2582, USA.

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Intra-individual variability of MFI for ζ-chain expression and the per cent of ζ-chain-negative cells in CD3+, CD4+ and CD8+ T cell subsets obtained from six normal donors (C1–6). The samples were drawn at three different time points 1 month apart. PBMC were cryopreserved and tested in the same assay to establish the biologic variation within each individual evaluated over a 3 month period. The solid line connects the median values. Of note, the percentages of ζ-chain-negative T cells are very low in normal controls, except for CD8+ T cells in C5.
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fig1: Intra-individual variability of MFI for ζ-chain expression and the per cent of ζ-chain-negative cells in CD3+, CD4+ and CD8+ T cell subsets obtained from six normal donors (C1–6). The samples were drawn at three different time points 1 month apart. PBMC were cryopreserved and tested in the same assay to establish the biologic variation within each individual evaluated over a 3 month period. The solid line connects the median values. Of note, the percentages of ζ-chain-negative T cells are very low in normal controls, except for CD8+ T cells in C5.

Mentions: In addition, cells of all six donors were repeatedly obtained at three time points separated by 1 month, and at each time point were tested together in one assay in order to determine biologic (intra-individual) variability in ζ-chain expression (Figure 1Figure 1


Recovery of zeta-chain expression and changes in spontaneous IL-10 production after PSA-based vaccines in patients with prostate cancer.

Meidenbauer N, Gooding W, Spitler L, Harris D, Whiteside TL - Br. J. Cancer (2002)

Intra-individual variability of MFI for ζ-chain expression and the per cent of ζ-chain-negative cells in CD3+, CD4+ and CD8+ T cell subsets obtained from six normal donors (C1–6). The samples were drawn at three different time points 1 month apart. PBMC were cryopreserved and tested in the same assay to establish the biologic variation within each individual evaluated over a 3 month period. The solid line connects the median values. Of note, the percentages of ζ-chain-negative T cells are very low in normal controls, except for CD8+ T cells in C5.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375179&req=5

fig1: Intra-individual variability of MFI for ζ-chain expression and the per cent of ζ-chain-negative cells in CD3+, CD4+ and CD8+ T cell subsets obtained from six normal donors (C1–6). The samples were drawn at three different time points 1 month apart. PBMC were cryopreserved and tested in the same assay to establish the biologic variation within each individual evaluated over a 3 month period. The solid line connects the median values. Of note, the percentages of ζ-chain-negative T cells are very low in normal controls, except for CD8+ T cells in C5.
Mentions: In addition, cells of all six donors were repeatedly obtained at three time points separated by 1 month, and at each time point were tested together in one assay in order to determine biologic (intra-individual) variability in ζ-chain expression (Figure 1Figure 1

Bottom Line: Prior to therapy, the patients were found to have significantly lower zeta-chain expression in circulating CD3(+) cells and a higher percentage of zeta-chain negative CD3(+) and CD4(+) cells than normal donors.Only one of 18 patients was a clinical responder.The vaccine had stimulatory effects on the patients' immune system, but post-vaccine immune recovery could not be correlated to progression-free survival in this small cohort of patients with prostate cancer.

View Article: PubMed Central - PubMed

Affiliation: University of Pittsburgh Cancer Institute, W1041 Biomedical Science Tower, 211 Lothrop Street, Pittsburgh, Pennsylvania, PA 15213-2582, USA.

Show MeSH
Related in: MedlinePlus