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Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.

Passalidou E, Trivella M, Singh N, Ferguson M, Hu J, Cesario A, Granone P, Nicholson AG, Goldstraw P, Ratcliffe C, Tetlow M, Leigh I, Harris AL, Gatter KC, Pezzella F - Br. J. Cancer (2002)

Bottom Line: In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative.Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%).We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: 3rd Department of Respiratory Medicine, Sismanogleiou Hospital, Sismanogleiou 1, PC 15126 Athens, Greece.

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Early papillary pattern (frozen sections). (A) (×40) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. The alveolar pattern appears preserved throughout the tumour. (B) (×100) Haematoxilin and Eosin (C) (×100) CD31 (in brown, the nuclei are counter stained with haematoxilin (blue)) and (D) (×100) CD34 (in brown, the nuclei are counter stained with haematoxilin (blue)). Within these micropapillae a small vessel (arrow), positive for CD31 and CD34, is present. (E) (×100) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. A new vessel CD31 positive but LH39 negative (arrow) is present in the stalk of this micropapilla. (F) (×100) Staining for αVβ3 (in brown). The nuclei are counter stained with haematoxilin (blue). An αVβ3 positive microvessel (arrow) within an early papilla.
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fig6: Early papillary pattern (frozen sections). (A) (×40) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. The alveolar pattern appears preserved throughout the tumour. (B) (×100) Haematoxilin and Eosin (C) (×100) CD31 (in brown, the nuclei are counter stained with haematoxilin (blue)) and (D) (×100) CD34 (in brown, the nuclei are counter stained with haematoxilin (blue)). Within these micropapillae a small vessel (arrow), positive for CD31 and CD34, is present. (E) (×100) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. A new vessel CD31 positive but LH39 negative (arrow) is present in the stalk of this micropapilla. (F) (×100) Staining for αVβ3 (in brown). The nuclei are counter stained with haematoxilin (blue). An αVβ3 positive microvessel (arrow) within an early papilla.

Mentions: A different pattern of angiogenic switch is instead observed in the remaining three cases of tumours. These cases had a papillary pattern (Pezzella et al, 1997). However the papillary component accounts for the minority of the tumour so we have termed it ‘early papillary’. As shown in Figure 6Figure 6


Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.

Passalidou E, Trivella M, Singh N, Ferguson M, Hu J, Cesario A, Granone P, Nicholson AG, Goldstraw P, Ratcliffe C, Tetlow M, Leigh I, Harris AL, Gatter KC, Pezzella F - Br. J. Cancer (2002)

Early papillary pattern (frozen sections). (A) (×40) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. The alveolar pattern appears preserved throughout the tumour. (B) (×100) Haematoxilin and Eosin (C) (×100) CD31 (in brown, the nuclei are counter stained with haematoxilin (blue)) and (D) (×100) CD34 (in brown, the nuclei are counter stained with haematoxilin (blue)). Within these micropapillae a small vessel (arrow), positive for CD31 and CD34, is present. (E) (×100) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. A new vessel CD31 positive but LH39 negative (arrow) is present in the stalk of this micropapilla. (F) (×100) Staining for αVβ3 (in brown). The nuclei are counter stained with haematoxilin (blue). An αVβ3 positive microvessel (arrow) within an early papilla.
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Related In: Results  -  Collection

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fig6: Early papillary pattern (frozen sections). (A) (×40) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. The alveolar pattern appears preserved throughout the tumour. (B) (×100) Haematoxilin and Eosin (C) (×100) CD31 (in brown, the nuclei are counter stained with haematoxilin (blue)) and (D) (×100) CD34 (in brown, the nuclei are counter stained with haematoxilin (blue)). Within these micropapillae a small vessel (arrow), positive for CD31 and CD34, is present. (E) (×100) Double immunostaining for LH39 (in brown) and CD31 (in blue). No counter staining was done. A new vessel CD31 positive but LH39 negative (arrow) is present in the stalk of this micropapilla. (F) (×100) Staining for αVβ3 (in brown). The nuclei are counter stained with haematoxilin (blue). An αVβ3 positive microvessel (arrow) within an early papilla.
Mentions: A different pattern of angiogenic switch is instead observed in the remaining three cases of tumours. These cases had a papillary pattern (Pezzella et al, 1997). However the papillary component accounts for the minority of the tumour so we have termed it ‘early papillary’. As shown in Figure 6Figure 6

Bottom Line: In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative.Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%).We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: 3rd Department of Respiratory Medicine, Sismanogleiou Hospital, Sismanogleiou 1, PC 15126 Athens, Greece.

Show MeSH
Related in: MedlinePlus