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Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.

Passalidou E, Trivella M, Singh N, Ferguson M, Hu J, Cesario A, Granone P, Nicholson AG, Goldstraw P, Ratcliffe C, Tetlow M, Leigh I, Harris AL, Gatter KC, Pezzella F - Br. J. Cancer (2002)

Bottom Line: In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative.Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%).We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: 3rd Department of Respiratory Medicine, Sismanogleiou Hospital, Sismanogleiou 1, PC 15126 Athens, Greece.

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A non-angiogenic tumour (frozen sections). (A) (×100) (low magnification) and (B) (×400) double immunostaining for LH39 (in brown) and CD31 (in blue); no counterstaining was done. All the vessels express LH39 in the basal membrane. (C) (×100), (D) (×400) Immunostaining for αVβ3 (in brown); the nuclei are counter stained with haematoxilin (blue). A variable positivity on the endothelial cells is present as seen in normal lung (Figure 1).
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fig2: A non-angiogenic tumour (frozen sections). (A) (×100) (low magnification) and (B) (×400) double immunostaining for LH39 (in brown) and CD31 (in blue); no counterstaining was done. All the vessels express LH39 in the basal membrane. (C) (×100), (D) (×400) Immunostaining for αVβ3 (in brown); the nuclei are counter stained with haematoxilin (blue). A variable positivity on the endothelial cells is present as seen in normal lung (Figure 1).

Mentions: In the nine putative non-angiogenic cases investigated, all the intratumoral vessels showed a basal membrane positive for LH39. The endothelial cells were negative, weakly stained or even focally strongly positive, with anti αVβ3 antibody, in a fashion similar to that observed in normal lung (Figure 2Figure 2


Vascular phenotype in angiogenic and non-angiogenic lung non-small cell carcinomas.

Passalidou E, Trivella M, Singh N, Ferguson M, Hu J, Cesario A, Granone P, Nicholson AG, Goldstraw P, Ratcliffe C, Tetlow M, Leigh I, Harris AL, Gatter KC, Pezzella F - Br. J. Cancer (2002)

A non-angiogenic tumour (frozen sections). (A) (×100) (low magnification) and (B) (×400) double immunostaining for LH39 (in brown) and CD31 (in blue); no counterstaining was done. All the vessels express LH39 in the basal membrane. (C) (×100), (D) (×400) Immunostaining for αVβ3 (in brown); the nuclei are counter stained with haematoxilin (blue). A variable positivity on the endothelial cells is present as seen in normal lung (Figure 1).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375177&req=5

fig2: A non-angiogenic tumour (frozen sections). (A) (×100) (low magnification) and (B) (×400) double immunostaining for LH39 (in brown) and CD31 (in blue); no counterstaining was done. All the vessels express LH39 in the basal membrane. (C) (×100), (D) (×400) Immunostaining for αVβ3 (in brown); the nuclei are counter stained with haematoxilin (blue). A variable positivity on the endothelial cells is present as seen in normal lung (Figure 1).
Mentions: In the nine putative non-angiogenic cases investigated, all the intratumoral vessels showed a basal membrane positive for LH39. The endothelial cells were negative, weakly stained or even focally strongly positive, with anti αVβ3 antibody, in a fashion similar to that observed in normal lung (Figure 2Figure 2

Bottom Line: In the nine putative non-angiogenic cases examined, the vascular phenotype of all the vessels was the same as that of alveolar vessels in normal lung: LH39 positive and alphaVbeta3 variable or negative.Instead in 104 angiogenic tumours examined, only a minority of vessels (mean 13.1%; range 0--60%) expressed LH39, while alphaVbeta3 (in 45 cases) was strongly expressed on many vessels (mean 55.5%; range 5--90%).We conclude that in putative non-angiogenic tumours the vascular phenotype is that of normal vessels and there is no neo-angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: 3rd Department of Respiratory Medicine, Sismanogleiou Hospital, Sismanogleiou 1, PC 15126 Athens, Greece.

Show MeSH
Related in: MedlinePlus