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IL-4 receptors on human medulloblastoma tumours serve as a sensitive target for a circular permuted IL-4-Pseudomonas exotoxin fusion protein.

Joshi BH, Leland P, Silber J, Kreitman RJ, Pastan I, Berger M, Puri RK - Br. J. Cancer (2002)

Bottom Line: These cells expressed IL-4Ralpha (also known as IL-4Rbeta) and IL-13Ralpha1 (also known as IL-13Ralpha') chains, however common gamma(c), a component of the interleukin-4 receptor system in immune cells was not detected.None of these cell lines showed any change in proliferation upon treatment with exogenous IL-4.These studies establish the interleukin-4 receptor as a medulloblastoma-associated target for possible tumour-directed cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Tumour Biology, Division of Cellular and Gene Therapies, Center for Biologics, Evaluation and Research, Food and Drug Administration, NIH Building 29B, Room 2NN10, 29 Lincoln Dr., Bethesda, Maryland, MD 20892, USA.

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Cytotoxicity of cpIL-4-PE against medullobalstoma cell lines. Ten thousand cells were cultured with various concentrations of cpIL4-PE. Cells were then pulsed with 1 μCi [3H]-Leucine and cell-associated radioactivity was measured with a Beta Plate Counter. The results are shown as mean ±s.d. of quadruplicate determinations and the experiment was repeated two times.
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fig3: Cytotoxicity of cpIL-4-PE against medullobalstoma cell lines. Ten thousand cells were cultured with various concentrations of cpIL4-PE. Cells were then pulsed with 1 μCi [3H]-Leucine and cell-associated radioactivity was measured with a Beta Plate Counter. The results are shown as mean ±s.d. of quadruplicate determinations and the experiment was repeated two times.

Mentions: As shown in Figure 3Figure 3


IL-4 receptors on human medulloblastoma tumours serve as a sensitive target for a circular permuted IL-4-Pseudomonas exotoxin fusion protein.

Joshi BH, Leland P, Silber J, Kreitman RJ, Pastan I, Berger M, Puri RK - Br. J. Cancer (2002)

Cytotoxicity of cpIL-4-PE against medullobalstoma cell lines. Ten thousand cells were cultured with various concentrations of cpIL4-PE. Cells were then pulsed with 1 μCi [3H]-Leucine and cell-associated radioactivity was measured with a Beta Plate Counter. The results are shown as mean ±s.d. of quadruplicate determinations and the experiment was repeated two times.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375174&req=5

fig3: Cytotoxicity of cpIL-4-PE against medullobalstoma cell lines. Ten thousand cells were cultured with various concentrations of cpIL4-PE. Cells were then pulsed with 1 μCi [3H]-Leucine and cell-associated radioactivity was measured with a Beta Plate Counter. The results are shown as mean ±s.d. of quadruplicate determinations and the experiment was repeated two times.
Mentions: As shown in Figure 3Figure 3

Bottom Line: These cells expressed IL-4Ralpha (also known as IL-4Rbeta) and IL-13Ralpha1 (also known as IL-13Ralpha') chains, however common gamma(c), a component of the interleukin-4 receptor system in immune cells was not detected.None of these cell lines showed any change in proliferation upon treatment with exogenous IL-4.These studies establish the interleukin-4 receptor as a medulloblastoma-associated target for possible tumour-directed cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Molecular Tumour Biology, Division of Cellular and Gene Therapies, Center for Biologics, Evaluation and Research, Food and Drug Administration, NIH Building 29B, Room 2NN10, 29 Lincoln Dr., Bethesda, Maryland, MD 20892, USA.

Show MeSH
Related in: MedlinePlus