Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix.
Bottom Line: Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein.When employed for RNA--RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages.In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions.
Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.Show MeSH
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Mentions: For in situ hybridization studies with clone G30CC as probe, positive signals were mainly observed in the cytoplasm (Figure 4). Expression of clone G30CC was consistently detected in cervical carcinomas of FIGO stages 1B, 2A, 2B and 3B (Figure 4). Although the G30CC anti-sense probe did not hybridize to the para-epithelial cell layers of matched normal tissues obtained from FIGO stages 1B and 2A cervical cancer patients, specific hybridization could be detected within the immature basal epithelial cell layers (Figure 4). More significantly, when the matched normal tissues obtained from FIGO stages 2B and 3B cervical cancer patients were studied with the G30CC anti-sense probes, strong hybridization signals could be obtained in the immature epithelial cells of histological normal tissue sections (Figure 4). Conversely, G30CC expression could not be detected when normal tissue sections collected from non-cervical cancer patients were tested (Figure 5Figure 5
Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.