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Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix.

Cheng Q, Lau WM, Chew SH, Ho TH, Tay SK, Hui KM - Br. J. Cancer (2002)

Bottom Line: Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein.When employed for RNA--RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages.In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.

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Expression of G30CC in normal epithelium of tissue biopsies obtained from four different non-cervical cancer patients via RNA–RNA in situ hybridization analysis. Sub-clones with the G30CC gene fragment inserted in opposite orientation were selected for the synthesis of the sense and anti-sense hybridization probes. The magnification shown was 600×.
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fig5: Expression of G30CC in normal epithelium of tissue biopsies obtained from four different non-cervical cancer patients via RNA–RNA in situ hybridization analysis. Sub-clones with the G30CC gene fragment inserted in opposite orientation were selected for the synthesis of the sense and anti-sense hybridization probes. The magnification shown was 600×.

Mentions: For in situ hybridization studies with clone G30CC as probe, positive signals were mainly observed in the cytoplasm (Figure 4). Expression of clone G30CC was consistently detected in cervical carcinomas of FIGO stages 1B, 2A, 2B and 3B (Figure 4). Although the G30CC anti-sense probe did not hybridize to the para-epithelial cell layers of matched normal tissues obtained from FIGO stages 1B and 2A cervical cancer patients, specific hybridization could be detected within the immature basal epithelial cell layers (Figure 4). More significantly, when the matched normal tissues obtained from FIGO stages 2B and 3B cervical cancer patients were studied with the G30CC anti-sense probes, strong hybridization signals could be obtained in the immature epithelial cells of histological normal tissue sections (Figure 4). Conversely, G30CC expression could not be detected when normal tissue sections collected from non-cervical cancer patients were tested (Figure 5Figure 5


Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix.

Cheng Q, Lau WM, Chew SH, Ho TH, Tay SK, Hui KM - Br. J. Cancer (2002)

Expression of G30CC in normal epithelium of tissue biopsies obtained from four different non-cervical cancer patients via RNA–RNA in situ hybridization analysis. Sub-clones with the G30CC gene fragment inserted in opposite orientation were selected for the synthesis of the sense and anti-sense hybridization probes. The magnification shown was 600×.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375172&req=5

fig5: Expression of G30CC in normal epithelium of tissue biopsies obtained from four different non-cervical cancer patients via RNA–RNA in situ hybridization analysis. Sub-clones with the G30CC gene fragment inserted in opposite orientation were selected for the synthesis of the sense and anti-sense hybridization probes. The magnification shown was 600×.
Mentions: For in situ hybridization studies with clone G30CC as probe, positive signals were mainly observed in the cytoplasm (Figure 4). Expression of clone G30CC was consistently detected in cervical carcinomas of FIGO stages 1B, 2A, 2B and 3B (Figure 4). Although the G30CC anti-sense probe did not hybridize to the para-epithelial cell layers of matched normal tissues obtained from FIGO stages 1B and 2A cervical cancer patients, specific hybridization could be detected within the immature basal epithelial cell layers (Figure 4). More significantly, when the matched normal tissues obtained from FIGO stages 2B and 3B cervical cancer patients were studied with the G30CC anti-sense probes, strong hybridization signals could be obtained in the immature epithelial cells of histological normal tissue sections (Figure 4). Conversely, G30CC expression could not be detected when normal tissue sections collected from non-cervical cancer patients were tested (Figure 5Figure 5

Bottom Line: Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein.When employed for RNA--RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages.In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.

Show MeSH
Related in: MedlinePlus