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Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix.

Cheng Q, Lau WM, Chew SH, Ho TH, Tay SK, Hui KM - Br. J. Cancer (2002)

Bottom Line: Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein.When employed for RNA--RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages.In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.

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Northern blot analysis employing clones G32C4B, G30CC, G30Ca, G31C5G, G30CI, G32C2B and G31C5E as probes with total RNA obtained from human squamous cell cervical carcinoma biopsies of different FIGO stages and their corresponding adjacent normal tissues. The summary table shows the folds of increase of the cloned cDNA fragments in the various tumour biopsies in comparison to their matched normal counterparts after normalization to G3PDH mRNA.
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fig3: Northern blot analysis employing clones G32C4B, G30CC, G30Ca, G31C5G, G30CI, G32C2B and G31C5E as probes with total RNA obtained from human squamous cell cervical carcinoma biopsies of different FIGO stages and their corresponding adjacent normal tissues. The summary table shows the folds of increase of the cloned cDNA fragments in the various tumour biopsies in comparison to their matched normal counterparts after normalization to G3PDH mRNA.

Mentions: To determine if the level of gene expression of some of the cloned genes correlate with the tumorigenicity of human squamous cell cervical cancer, Northern blot analyses were performed. mRNA from cervical cancer biopsies of FIGO stages 1B (four patients), 2A (three patients), 2B (two patients), and 3B (four patients), along with their matched normal tissue biopsies were isolated. The level of expression of the cloned genes identified in this study in cervical cancer biopsies of various FIGO stages was compared to that of their matched normal tissues. Our results demonstrated that expression of clone G30CC was consistently upregulated in the cervical cancer tissue biopsies examined (Figure 3Figure 3


Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix.

Cheng Q, Lau WM, Chew SH, Ho TH, Tay SK, Hui KM - Br. J. Cancer (2002)

Northern blot analysis employing clones G32C4B, G30CC, G30Ca, G31C5G, G30CI, G32C2B and G31C5E as probes with total RNA obtained from human squamous cell cervical carcinoma biopsies of different FIGO stages and their corresponding adjacent normal tissues. The summary table shows the folds of increase of the cloned cDNA fragments in the various tumour biopsies in comparison to their matched normal counterparts after normalization to G3PDH mRNA.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2375172&req=5

fig3: Northern blot analysis employing clones G32C4B, G30CC, G30Ca, G31C5G, G30CI, G32C2B and G31C5E as probes with total RNA obtained from human squamous cell cervical carcinoma biopsies of different FIGO stages and their corresponding adjacent normal tissues. The summary table shows the folds of increase of the cloned cDNA fragments in the various tumour biopsies in comparison to their matched normal counterparts after normalization to G3PDH mRNA.
Mentions: To determine if the level of gene expression of some of the cloned genes correlate with the tumorigenicity of human squamous cell cervical cancer, Northern blot analyses were performed. mRNA from cervical cancer biopsies of FIGO stages 1B (four patients), 2A (three patients), 2B (two patients), and 3B (four patients), along with their matched normal tissue biopsies were isolated. The level of expression of the cloned genes identified in this study in cervical cancer biopsies of various FIGO stages was compared to that of their matched normal tissues. Our results demonstrated that expression of clone G30CC was consistently upregulated in the cervical cancer tissue biopsies examined (Figure 3Figure 3

Bottom Line: Of particular interest is clone G30CC that has been identified to be the gene that encodes S12 ribosomal protein.When employed for RNA--RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages.In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Gene Structure & Expression, Division of Cellular and Molecular Research, National Cancer Centre, 11 Hospital Drive, 169610 Singapore.

Show MeSH
Related in: MedlinePlus