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Lymphangiogenesis in myocardial remodelling after infarction.

Ishikawa Y, Akishima-Fukasawa Y, Ito K, Akasaka Y, Tanaka M, Shimokawa R, Kimura-Matsumoto M, Morita H, Sato S, Kamata I, Ishii T - Histopathology (2007)

Bottom Line: BD decreased with advance of myocardial necrosis, subsequently increased at the early stage of granulation and thereafter decreased with the progression of scar formation.Vascular endothelial growth factor-C was consistently expressed in viable cardiomyocytes around the lesion in all of these stages.In myocardial remodelling after MI, lymphangiogenesis lags behind blood vessel angiogenesis; newly formed lymphatics may be involved mainly in the maturation of fibrosis and scar formation through the drainage of excessive proteins and fluid.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Toho University School of Medicine, Tokyo, Japan. yukio@med.toho-u.ac.jp

ABSTRACT

Aims: The lymphatic system is involved in fluid homeostasis of the cardiac interstitium, but lymphangiogenesis in myocardial remodelling has not previously been examined histopathologically. The aim was to investigate by D2-40 immunohistochemistry the sequential changes in lymphatic distribution in the process of myocardial remodelling after myocardial infarction (MI).

Methods and results: Myocardial tissues in various phases of healing after MI were obtained from 40 autopsied hearts. D2-40+ lymphatic vessel density (LD) and CD34+ blood vessel density (BD) in the lesion were determined. BD decreased with advance of myocardial necrosis, subsequently increased at the early stage of granulation and thereafter decreased with the progression of scar formation. In contrast, lymphatic vessels were not detected in lesions with coagulation necrosis, and newly formed lymphatics first appeared in the early stages of granulation. A subsequent increase in LD was demonstrated in the late stages of granulation, and lymphatics remained up to the scar phase. Vascular endothelial growth factor-C was consistently expressed in viable cardiomyocytes around the lesion in all of these stages.

Conclusion: In myocardial remodelling after MI, lymphangiogenesis lags behind blood vessel angiogenesis; newly formed lymphatics may be involved mainly in the maturation of fibrosis and scar formation through the drainage of excessive proteins and fluid.

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Related in: MedlinePlus

Immunohistochemistry in Stages VI (a) and VII (b–d)a, The upper half of the figure shows the lesion with fibrosis (Stage VI), in which D2-40+ lymphatics are scattered adjacent to the viable cardiomyocytes (the lower half of the figure). Their lumens are dilated. b, In Stage VII, showing scar formation, D2-40+ lymphatics are scattered in the periphery of the lesion. c, In the scar, muscular blood vessels immunopositive for smooth muscle actin are scattered. d, Cardiomyocytes around the scar are positive for vascular endothelial growth factor-C.
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fig03: Immunohistochemistry in Stages VI (a) and VII (b–d)a, The upper half of the figure shows the lesion with fibrosis (Stage VI), in which D2-40+ lymphatics are scattered adjacent to the viable cardiomyocytes (the lower half of the figure). Their lumens are dilated. b, In Stage VII, showing scar formation, D2-40+ lymphatics are scattered in the periphery of the lesion. c, In the scar, muscular blood vessels immunopositive for smooth muscle actin are scattered. d, Cardiomyocytes around the scar are positive for vascular endothelial growth factor-C.

Mentions: In these lesions, dilated lymphatics were observed in the peripheral region (Figure 3a). Lymphatic capillaries were also scattered in the lesion and tended to be distributed in the peripheral region.


Lymphangiogenesis in myocardial remodelling after infarction.

Ishikawa Y, Akishima-Fukasawa Y, Ito K, Akasaka Y, Tanaka M, Shimokawa R, Kimura-Matsumoto M, Morita H, Sato S, Kamata I, Ishii T - Histopathology (2007)

Immunohistochemistry in Stages VI (a) and VII (b–d)a, The upper half of the figure shows the lesion with fibrosis (Stage VI), in which D2-40+ lymphatics are scattered adjacent to the viable cardiomyocytes (the lower half of the figure). Their lumens are dilated. b, In Stage VII, showing scar formation, D2-40+ lymphatics are scattered in the periphery of the lesion. c, In the scar, muscular blood vessels immunopositive for smooth muscle actin are scattered. d, Cardiomyocytes around the scar are positive for vascular endothelial growth factor-C.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2366023&req=5

fig03: Immunohistochemistry in Stages VI (a) and VII (b–d)a, The upper half of the figure shows the lesion with fibrosis (Stage VI), in which D2-40+ lymphatics are scattered adjacent to the viable cardiomyocytes (the lower half of the figure). Their lumens are dilated. b, In Stage VII, showing scar formation, D2-40+ lymphatics are scattered in the periphery of the lesion. c, In the scar, muscular blood vessels immunopositive for smooth muscle actin are scattered. d, Cardiomyocytes around the scar are positive for vascular endothelial growth factor-C.
Mentions: In these lesions, dilated lymphatics were observed in the peripheral region (Figure 3a). Lymphatic capillaries were also scattered in the lesion and tended to be distributed in the peripheral region.

Bottom Line: BD decreased with advance of myocardial necrosis, subsequently increased at the early stage of granulation and thereafter decreased with the progression of scar formation.Vascular endothelial growth factor-C was consistently expressed in viable cardiomyocytes around the lesion in all of these stages.In myocardial remodelling after MI, lymphangiogenesis lags behind blood vessel angiogenesis; newly formed lymphatics may be involved mainly in the maturation of fibrosis and scar formation through the drainage of excessive proteins and fluid.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Toho University School of Medicine, Tokyo, Japan. yukio@med.toho-u.ac.jp

ABSTRACT

Aims: The lymphatic system is involved in fluid homeostasis of the cardiac interstitium, but lymphangiogenesis in myocardial remodelling has not previously been examined histopathologically. The aim was to investigate by D2-40 immunohistochemistry the sequential changes in lymphatic distribution in the process of myocardial remodelling after myocardial infarction (MI).

Methods and results: Myocardial tissues in various phases of healing after MI were obtained from 40 autopsied hearts. D2-40+ lymphatic vessel density (LD) and CD34+ blood vessel density (BD) in the lesion were determined. BD decreased with advance of myocardial necrosis, subsequently increased at the early stage of granulation and thereafter decreased with the progression of scar formation. In contrast, lymphatic vessels were not detected in lesions with coagulation necrosis, and newly formed lymphatics first appeared in the early stages of granulation. A subsequent increase in LD was demonstrated in the late stages of granulation, and lymphatics remained up to the scar phase. Vascular endothelial growth factor-C was consistently expressed in viable cardiomyocytes around the lesion in all of these stages.

Conclusion: In myocardial remodelling after MI, lymphangiogenesis lags behind blood vessel angiogenesis; newly formed lymphatics may be involved mainly in the maturation of fibrosis and scar formation through the drainage of excessive proteins and fluid.

Show MeSH
Related in: MedlinePlus