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Mycobacterium avium subspecies paratuberculosis is not associated with type-2 diabetes mellitus.

Rosu V, Ahmed N, Paccagnini D, Pacifico A, Zanetti S, Sechi LA - Ann. Clin. Microbiol. Antimicrob. (2008)

Bottom Line: Statistical analysis was performed with the Chi-square test with Yates' corrections.We observed insignificant levels of humoral antibodies against recombinant heparin binding haemagglutinin (HbHA), glycosyl transferase (Gsd) and MAP whole cell lysate in the blood of subjects with T2DM as compared to healthy controls.We found no obvious association of MAP with the incidence of T2DM in Sardinian patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Scienze Biomediche, Sezione di Microbiologia clinica e sperimentale, viale San Pietro 43 b 07100 Sassari, Italy. v.rosu@email.it

ABSTRACT

Background: The role of pathogenic mycobacteria in diabetes has been a focus of speculation since a decade without any meaningful insights into the mechanism of diabetes causation vis a vis mycobacterial factors. Two of our studies based on PCR identification of mycobacterial DNA and detection of antibodies specific to the recombinant antigens and whole cell lysates of the Mycobacterium avium subsp. paratuberculosis (MAP) shown a clear association of MAP with the presence of type 1 diabetes mellitus (T1DM).

Methods: In this study, we sought to investigate if or not type 2 diabetes (T2DM) patients harbour humoral responses to MAP. Using three different MAP antigen preparations, humoral antibody profiles were estimated for 57 T2DM patients and 57 healthy controls. Statistical analysis was performed with the Chi-square test with Yates' corrections.

Results: We observed insignificant levels of humoral antibodies against recombinant heparin binding haemagglutinin (HbHA), glycosyl transferase (Gsd) and MAP whole cell lysate in the blood of subjects with T2DM as compared to healthy controls.

Conclusion: We found no obvious association of MAP with the incidence of T2DM in Sardinian patients.

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Related in: MedlinePlus

Evaluation of serum samples from diabetic type II patients (left column) and healthy donors (right column) against HbHA recombinant protein (A), Gsd recombinant protein (B) and MAP lysate (C). Data are presented as values of OD405 observed following ELISA, as described in the text. Data from a representative experiment out of three are shown. The median value for each group is indicated by a dark solid horizontal line.
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Figure 1: Evaluation of serum samples from diabetic type II patients (left column) and healthy donors (right column) against HbHA recombinant protein (A), Gsd recombinant protein (B) and MAP lysate (C). Data are presented as values of OD405 observed following ELISA, as described in the text. Data from a representative experiment out of three are shown. The median value for each group is indicated by a dark solid horizontal line.

Mentions: ELISA values were interpreted in the form of optical density (OD) and the values were plotted as shown (Figure 1). An arbitrary cut-off was established as in the previous work [11]. HbHA antigen ELISA titres with a cut-off titre value 0.5 (Chi square equal to 0.000, P value equal to 0.9899); the Gsd protein cut-off value of 0.4 (Chi square equal to 0.154 with 1 degrees of freedom, P value equal to 0.6945); MAP lysate cut-off value of 0.5 (Chi square equal to 0.091 with 1 degrees of freedom, P value equal to 0.7627).


Mycobacterium avium subspecies paratuberculosis is not associated with type-2 diabetes mellitus.

Rosu V, Ahmed N, Paccagnini D, Pacifico A, Zanetti S, Sechi LA - Ann. Clin. Microbiol. Antimicrob. (2008)

Evaluation of serum samples from diabetic type II patients (left column) and healthy donors (right column) against HbHA recombinant protein (A), Gsd recombinant protein (B) and MAP lysate (C). Data are presented as values of OD405 observed following ELISA, as described in the text. Data from a representative experiment out of three are shown. The median value for each group is indicated by a dark solid horizontal line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2365959&req=5

Figure 1: Evaluation of serum samples from diabetic type II patients (left column) and healthy donors (right column) against HbHA recombinant protein (A), Gsd recombinant protein (B) and MAP lysate (C). Data are presented as values of OD405 observed following ELISA, as described in the text. Data from a representative experiment out of three are shown. The median value for each group is indicated by a dark solid horizontal line.
Mentions: ELISA values were interpreted in the form of optical density (OD) and the values were plotted as shown (Figure 1). An arbitrary cut-off was established as in the previous work [11]. HbHA antigen ELISA titres with a cut-off titre value 0.5 (Chi square equal to 0.000, P value equal to 0.9899); the Gsd protein cut-off value of 0.4 (Chi square equal to 0.154 with 1 degrees of freedom, P value equal to 0.6945); MAP lysate cut-off value of 0.5 (Chi square equal to 0.091 with 1 degrees of freedom, P value equal to 0.7627).

Bottom Line: Statistical analysis was performed with the Chi-square test with Yates' corrections.We observed insignificant levels of humoral antibodies against recombinant heparin binding haemagglutinin (HbHA), glycosyl transferase (Gsd) and MAP whole cell lysate in the blood of subjects with T2DM as compared to healthy controls.We found no obvious association of MAP with the incidence of T2DM in Sardinian patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dipartimento di Scienze Biomediche, Sezione di Microbiologia clinica e sperimentale, viale San Pietro 43 b 07100 Sassari, Italy. v.rosu@email.it

ABSTRACT

Background: The role of pathogenic mycobacteria in diabetes has been a focus of speculation since a decade without any meaningful insights into the mechanism of diabetes causation vis a vis mycobacterial factors. Two of our studies based on PCR identification of mycobacterial DNA and detection of antibodies specific to the recombinant antigens and whole cell lysates of the Mycobacterium avium subsp. paratuberculosis (MAP) shown a clear association of MAP with the presence of type 1 diabetes mellitus (T1DM).

Methods: In this study, we sought to investigate if or not type 2 diabetes (T2DM) patients harbour humoral responses to MAP. Using three different MAP antigen preparations, humoral antibody profiles were estimated for 57 T2DM patients and 57 healthy controls. Statistical analysis was performed with the Chi-square test with Yates' corrections.

Results: We observed insignificant levels of humoral antibodies against recombinant heparin binding haemagglutinin (HbHA), glycosyl transferase (Gsd) and MAP whole cell lysate in the blood of subjects with T2DM as compared to healthy controls.

Conclusion: We found no obvious association of MAP with the incidence of T2DM in Sardinian patients.

Show MeSH
Related in: MedlinePlus