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Hydrogenated fat diet intake during pregnancy and lactation modifies the PAI-1 gene expression in white adipose tissue of offspring in adult life.

Pisani LP, Oller do Nascimento CM, Bueno AA, Biz C, Albuquerque KT, Ribeiro EB, Oyama LM - Lipids Health Dis (2008)

Bottom Line: Blood and carcass as well as retroperitoneal, epididymal, and subcutaneous white adipose tissues were collected.Food intake and body weight were lower in TC and TT, and metabolic efficiency was reduced in TT.Ingestion of hydrogenated vegetable fat by the mother during gestation and lactation could promote deleterious consequences, even after the withdrawal of the causal factor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bioscience, São Paulo Federal University - UNIFESP, v, Ana Costa, Santos, São Paulo 11060-001, Brazil. lucianapisani@gmail.com

ABSTRACT
We examine whether feeding pregnant and lactating rats hydrogenated fats rich in trans fatty acids modifies the plasma lipid profiles and the expression of adipokines involved with insulin resistance and cardiovascular disease in their 90-day-old offspring. Pregnant and lactating Wistar rats were fed with either a control diet (C group) or one enriched with hydrogenated vegetable fat (T group). Upon weaning, the male pups were sorted into four groups: CC, mothers were receiving C and pups were kept on C; CT, mothers were receiving C and pups were fed with T; TT, mothers were receiving T and pups were kept on T; TC, mothers were receiving T and pups were fed with C. Pups' food intake and body weight were quantified weekly and the pups were killed at day 90 of life by decapitation. Blood and carcass as well as retroperitoneal, epididymal, and subcutaneous white adipose tissues were collected. Food intake and body weight were lower in TC and TT, and metabolic efficiency was reduced in TT. Offspring of TT and TC rats had increased white adipose tissue PAI-1 gene expression. Insulin receptor was higher in TT than other groups. Ingestion of hydrogenated vegetable fat by the mother during gestation and lactation could promote deleterious consequences, even after the withdrawal of the causal factor.

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(A) Body weight. (B) Total body weight gain. (C) Total food intake. (D) metabolic efficiency. CC, mothers fed C diet and offspring fed C diet; CT, mothers fed C diet and offspring fed T diet; TT, mothers fed T diet and offspring fed T diet; TC, mothers fed T diet and offspring fed C diet. The number of studied animals per each group is 10. Data are means ± SE of 10 determinations per group. *p < 0.05, TT compared with CC. #p < 0.05, TC compared with CC.
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Figure 1: (A) Body weight. (B) Total body weight gain. (C) Total food intake. (D) metabolic efficiency. CC, mothers fed C diet and offspring fed C diet; CT, mothers fed C diet and offspring fed T diet; TT, mothers fed T diet and offspring fed T diet; TC, mothers fed T diet and offspring fed C diet. The number of studied animals per each group is 10. Data are means ± SE of 10 determinations per group. *p < 0.05, TT compared with CC. #p < 0.05, TC compared with CC.

Mentions: The body weights of the pups at 21 days old did not differ between the C and T groups. However, both the group TC at the seventh week and the group TT at the fourth week after weaning (Figure 1A) had lower body weights compared with CC. The total body weight gain in CC was higher than TT, TC and CT (Figure 1B). Also, TT had increase in total body weight gain as compared to TC group (Figure 1B). However, the food intake of both TT and TC was lower than CC and CT (Figure 1C).


Hydrogenated fat diet intake during pregnancy and lactation modifies the PAI-1 gene expression in white adipose tissue of offspring in adult life.

Pisani LP, Oller do Nascimento CM, Bueno AA, Biz C, Albuquerque KT, Ribeiro EB, Oyama LM - Lipids Health Dis (2008)

(A) Body weight. (B) Total body weight gain. (C) Total food intake. (D) metabolic efficiency. CC, mothers fed C diet and offspring fed C diet; CT, mothers fed C diet and offspring fed T diet; TT, mothers fed T diet and offspring fed T diet; TC, mothers fed T diet and offspring fed C diet. The number of studied animals per each group is 10. Data are means ± SE of 10 determinations per group. *p < 0.05, TT compared with CC. #p < 0.05, TC compared with CC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2365943&req=5

Figure 1: (A) Body weight. (B) Total body weight gain. (C) Total food intake. (D) metabolic efficiency. CC, mothers fed C diet and offspring fed C diet; CT, mothers fed C diet and offspring fed T diet; TT, mothers fed T diet and offspring fed T diet; TC, mothers fed T diet and offspring fed C diet. The number of studied animals per each group is 10. Data are means ± SE of 10 determinations per group. *p < 0.05, TT compared with CC. #p < 0.05, TC compared with CC.
Mentions: The body weights of the pups at 21 days old did not differ between the C and T groups. However, both the group TC at the seventh week and the group TT at the fourth week after weaning (Figure 1A) had lower body weights compared with CC. The total body weight gain in CC was higher than TT, TC and CT (Figure 1B). Also, TT had increase in total body weight gain as compared to TC group (Figure 1B). However, the food intake of both TT and TC was lower than CC and CT (Figure 1C).

Bottom Line: Blood and carcass as well as retroperitoneal, epididymal, and subcutaneous white adipose tissues were collected.Food intake and body weight were lower in TC and TT, and metabolic efficiency was reduced in TT.Ingestion of hydrogenated vegetable fat by the mother during gestation and lactation could promote deleterious consequences, even after the withdrawal of the causal factor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Bioscience, São Paulo Federal University - UNIFESP, v, Ana Costa, Santos, São Paulo 11060-001, Brazil. lucianapisani@gmail.com

ABSTRACT
We examine whether feeding pregnant and lactating rats hydrogenated fats rich in trans fatty acids modifies the plasma lipid profiles and the expression of adipokines involved with insulin resistance and cardiovascular disease in their 90-day-old offspring. Pregnant and lactating Wistar rats were fed with either a control diet (C group) or one enriched with hydrogenated vegetable fat (T group). Upon weaning, the male pups were sorted into four groups: CC, mothers were receiving C and pups were kept on C; CT, mothers were receiving C and pups were fed with T; TT, mothers were receiving T and pups were kept on T; TC, mothers were receiving T and pups were fed with C. Pups' food intake and body weight were quantified weekly and the pups were killed at day 90 of life by decapitation. Blood and carcass as well as retroperitoneal, epididymal, and subcutaneous white adipose tissues were collected. Food intake and body weight were lower in TC and TT, and metabolic efficiency was reduced in TT. Offspring of TT and TC rats had increased white adipose tissue PAI-1 gene expression. Insulin receptor was higher in TT than other groups. Ingestion of hydrogenated vegetable fat by the mother during gestation and lactation could promote deleterious consequences, even after the withdrawal of the causal factor.

Show MeSH
Related in: MedlinePlus